C-reactive protein/serum amyloid P promotes pro-inflammatory function and induces M1-type polarization of monocytes/macrophages in mudskipper, Boleophthalmus pectinirostris
C-reactive protein/serum amyloid P promotes pro-inflammatory function and induces M1-type polarization of monocytes/macrophages in mudskipper, Boleophthalmus pectinirostris
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C反应蛋白/血清淀粉样蛋白P促进弹涂鱼、大弹涂鱼的促炎功能并诱导单核细胞/巨噬细胞的M1型极化
DOI：
10.1016/j.fsi.2019.09.021
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发表时间：
2019-11-01
影响因子：
4.7
通讯作者：
Chen，Jiong
中科院分区：
文献类型：
--
作者：
Cai，Shi-Yu;Nie，Li;Chen，Jiong
C反应蛋白（CRP）和血清淀粉样蛋白P（SAP）在哺乳动物吞噬细胞介导的先天免疫反应中发挥重要作用。已在哺乳动物中完成对CRP和SAP的深入研究；然而，此类研究，特别是与 CRP 和 SAP 功能相关的研究，在鱼类中很少见。在本研究中，在弹涂鱼（弹涂鱼）中鉴定出了 CRP/SAP（BpCRP/SAP）的同源物，该蛋白具有鱼类短五聚蛋白的典型特征。系统发育树分析表明BpCRP/SAP与弹涂鱼CRP/SAP-l3关系最密切。在所有测试组织中均检测到 BpCRP/SAP 转录本，其中在肝脏中观察到最高水平；免疫组织中的转录物和血清中的蛋白质表达是针对迟缓爱德华氏菌感染而诱导的。活性重组 BpCRP/SAP (rBpCRP/SAP) 能够增强单核细胞/巨噬细胞 (MO/MΦ) 中促炎细胞因子的 mRNA 表达并减弱抗炎细胞因子的 mRNA 表达。此外，大肠杆菌的吞噬作用和细菌杀灭作用。 rBpCRP/SAP 刺激增强了迟钝弹涂鱼 MO/MΦ。 rBpCRP/SAP还促进M1型MO/MΦ极化，但抑制M2型极化。总之，本研究描述了弹涂鱼中 BpCRP/SAP 的促炎功能。迟发性感染。
C-reactive protein (CRP) and serum amyloid P (SAP) play essential roles in the phagocytic cell-mediated innate immune response of mammals. In-depth studies into CRP and SAP have been completed in mammals; however, such studies, particularly those relating to the functions of CRP and SAP, are rare in fish species. In this study, a homolog of CRP/SAP (BpCRP/SAP) was identified in mudskipper (Boleophthalmus pectinirostris), which had the typical characteristics of a fish short pentraxin protein. Phylogenetic tree analysis revealed that BpCRP/SAP was most closely related to mudskipper CRP/SAP-l3. BpCRP/SAP transcripts were detected in all tested tissues, with the highest level observed in the liver; transcripts in the immune tissues and protein expression in the serum were induced in response toEdwardsiella tardainfection. The active recombinant BpCRP/SAP (rBpCRP/SAP) was able to augment the mRNA expression of pro-inflammatory cytokines and attenuate the mRNA expression of anti-inflammatory cytokines in monocytes/macrophages (MO/MΦ). In addition, phagocytosis and bacterial killing ofE. tardaby mudskipper MO/MΦ were boosted by rBpCRP/SAP stimulation. rBpCRP/SAP also promoted M1-type MO/MΦ polarization, but inhibited M2-type polarization. In conclusion, the present research describes the pro-inflammatory function of BpCRP/SAP in mudskipper againstE. tardainfection.