Characterization of Breast Cancer Progression in the Rat
Characterization of Breast Cancer Progression in the Rat
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DOI：
10.1196/annals.1414.018
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发表时间：
2008-01-01
期刊：
影响因子：
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通讯作者：
Karam, Sherif M.
中科院分区：
文献类型：
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作者：
Al-Dhaheri, Wafa S.;Hassouna, Imam;Karam, Sherif M.
乳腺癌的发病率在全球范围内持续上升。这一增长趋势部分归因于相当多的病例与环境因素有关，部分归因于关于乳腺癌发生过程中发生的早期变化的信息很少。为了表征这些早期细胞变化中的一些，使用单次胃内剂量的环境致癌物7，12-二甲基苯并[a]蒽（DMBA：80 mg/kg体重）在雌性大鼠中诱导乳腺癌。对照组和DMBA处理组大鼠乳腺组织进行常规组织病理学检查和免疫组化分析，使用增殖细胞核抗原（PCNA）的特异性抗体。显微镜检查DMBA处理的大鼠的所有乳腺显示了广泛的癌前阶段，除了良好的特征良性和恶性肿瘤的发展。第一阶段的特征是末端导管轻微扩张，死细胞聚集。这被称为细胞死亡的阶段。然后是增生、异型增生和原位癌。PCNA在这些癌前病变的免疫组化定位显示，由标记指数的PCNA逐渐增加，然后由最初的下降。总之，DMBA处理的大鼠提供了一个有用的模型，解剖乳腺癌发生的多步骤过程中发生的早期变化。
The incidence of breast cancer is continuously increasing worldwide. This increasing trend is attributed partly to the fact that a considerable number of cases are related to environmental factors and partly to the little information available on the early changes that occur during mammary gland carcinogenesis. To characterize some of these early cellular changes, breast cancer was induced in female rats using a single intragastric dose of the environmental carcinogen 7,12-dimethylbenz[a]anthracene (DMBA: 80 mg/kg body weight). Mammary gland tissues of control and DMBA-treated rats were processed for routine histopathological examination and immunohistochemical analysis using an antibody specific for the proliferating cell nuclear antigen (PCNA). Microscopic examination of all mammary glands of DMBA-treated rats revealed a wide range of preneoplastic stages in addition to the well-characterized benign and malignant tumors that developed. The first stage was characterized by slightly dilated terminal ducts with accumulation of dead cells. This was designated the stage of cell death. Then, stages of hyperplasia, dysplasia, and carcinoma in situ followed. Immunohistochemical localization of PCNA in these preneoplastic lesions revealed an initial decrease followed by a gradual increase in the labeling index of PCNA. In conclusion, the DMBA-treated rats provide a useful model to dissect the early changes that occur during the multistep process of mammary gland carcinogenesis.