The calculated stability of DNA duplexes containing an oxidized guanine lesion pairing with guanine
The calculated stability of DNA duplexes containing an oxidized guanine lesion pairing with guanine
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含有氧化鸟嘌呤损伤与鸟嘌呤配对的 DNA 双链体的计算稳定性
DOI：
10.3390/ecsoc-17-e011
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
H. Miyazawa
中科院分区：
文献类型：
--
作者：
Masayo Suzuki;K. Kino;Masayuki Morikawa;Taihei Watanabe;Eriko Asada;Takanobu Kobayashi;H. Miyazawa
DNA受到各种氧化应激的损伤。氧化的DNA会增加突变和癌症的风险。鸟嘌呤由于其低氧化电位而对几种氧化应激高度敏感。已知2，2，4-三氨基-5（2 H）-恶唑酮（Oz）、亚氨基尿囊素（Ia）和螺亚氨基二乙内酰脲（Sp）是氧化鸟嘌呤病变。这些氧化的碱基可以与鸟嘌呤配对并引起G：C-C：G颠换。虽然我们以前的结果表明，与Ia或Sp相反相比，Oz对鸟嘌呤的掺入更有效（K. Kino等人，ChemBioChem，2009），通过从头计算，Oz：G碱基对的稳定性低于Ia：G或Sp：G碱基对（M. Suzuki等人，Molecules，2012）。Oz：G形成两个氢键并且是平面的，而Ia：G和Sp：G具有三个氢键并且是非平面的。这种差异可能是由于Ia：G和Sp：G含有sp3碳而不是Oz：G。由于堆叠平面碱基对的DNA显着提高DNA双链体的稳定性，我们调查是否可以占在这项研究中的堆叠效应的氧化损伤的DNA双链体的稳定性。含Oz：G的DNA双链体结构最稳定。我们的数据与以前的结果一致，即跨Oz的经损伤合成比Ia或Sp更有效。
DNA is damaged by various oxidative stresses. Oxidized DNA can increase mutations and the risk of cancer. Guanine is highly sensitive to several oxidative stresses due to its low oxidation potential. It is known that 2,2,4-triamino-5(2H)-oxazolone (Oz), iminoallantoin (Ia) and spiroiminodihydantoin (Sp) are oxidized guanine lesions. These oxidized bases can be paired with guanine and cause G:C-C:G transversions. Although our previous results showed more effective incorporation of guanine opposite Oz compared to that opposite Ia or Sp (K. Kino et al., ChemBioChem, 2009), Oz:G base pair was less stable than Ia:G or Sp:G base pair by ab initio calculation (M. Suzuki et al., Molecules, 2012). Oz:G forms two hydrogen bonds and is planar, while Ia:G and Sp:G have three hydrogen bonds and are nonplanar. This difference can be due to the fact that Ia:G and Sp:G contain a sp3 carbon but not Oz:G. Since stacking planar base pairs in DNA significantly enhances the stability of DNA duplex, we investigate whether the stability of DNA duplex containing the oxidized lesion can be accounted for by the stacking effect in this study. The structure of DNA duplex containing Oz:G was the most stable. This our data agreed with the previous results that translesion synthesis across Oz was more effective than Ia or Sp.
影响因子：
4.1
作者：
Luo, WC;Muller, JG;Burrows, CJ
通讯作者：
Burrows, CJ