Raman spectroscopy of single human tumour cells exposed to ionizing radiation in vitro
Raman spectroscopy of single human tumour cells exposed to ionizing radiation in vitro
复制标题
DOI：
10.1088/0031-9155/56/1/002
复制
发表时间：
2011-01-07
影响因子：
3.5
通讯作者：
Jirasek, A.
中科院分区：
文献类型：
--
作者：
Matthews, Q.;Brolo, A. G.;Jirasek, A.
这项工作研究了拉曼光谱(RS)研究电离辐射对单个人类肿瘤细胞的影响的能力。前列腺肿瘤细胞(DU145细胞系)在体外培养，照射剂量为15-50Gy6 mV的单次光子。单细胞拉曼光谱是从辐照和未辐照的培养物中获得的，最多在辐照后5天。主成分分析被用来区分独特的辐射引起的光谱变化和由细胞周期差异和其他已知因素引起的光谱变异性的固有来源。我们观察到独特的辐射诱导的光谱变化，这与辐射剂量和辐射后的孵化时间都相关。辐射引起的光谱变化是由于照射和未照射的细胞在脂类、核酸、氨基酸和构象蛋白质结构上的生化差异引起的。据我们所知，这项研究是第一次使用RS来观察辐射诱导的单细胞生化差异，也是第一次使用振动光谱来观察独立于同时发生的细胞周期或细胞死亡相关过程的辐射诱导的单细胞生化差异。
This work investigates the capability of Raman spectroscopy (RS) to study the effects of ionizing radiation on single human tumour cells. Prostate tumour cells (cell line DU145) are cultured in vitro and irradiated to doses between 15 and 50 Gy with single fractions of 6 MV photons. Single-cell Raman spectra are acquired from irradiated and unirradiated cultures up to 5 days post-irradiation. Principal component analysis is used to distinguish the uniquely radiation-induced spectral changes from inherent sources of spectral variability arising from cell cycle differences and other known factors. We observe uniquely radiation-induced spectral changes which are correlated with both the irradiated dose and the incubation time post-irradiation. The spectral changes induced by radiation arise from biochemical differences in lipids, nucleic acids, amino acids and conformational protein structures between irradiated and unirradiated cells. To our knowledge, this study is the first use of RS to observe radiation-induced biochemical differences in single cells, and is the first use of vibrational spectroscopy to observe uniquely radiation-induced biochemical differences in single cells independent of concurrent cell-cycle-or cell-death-related processes.