DosR-regulon genes induction in Mycobacterium bovis BCG under aerobic conditions
DosR-regulon genes induction in Mycobacterium bovis BCG under aerobic conditions
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DOI：
10.1016/j.tube.2010.04.001
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发表时间：
2010-05-01
期刊：
影响因子：
3.2
通讯作者：
Schoolnik, Gary K.
中科院分区：
文献类型：
--
作者：
Flores-Valdez, Mario Alberto;Schoolnik, Gary K.
在这份报告中，我们证明了在有氧条件下，表达hsp60驱动的第二个副本的低氧相关的转录调节因子DosR的牛分枝杆菌卡介苗增加2倍或更多的表达的48个基因属于DosR调节子，包括潜伏抗原Rv1733c，Rv2029，Rv2627和Rv2628。在这些条件下的DosR的表达略有延迟在体外生长，但没有促进非复制状态，而不是微氧和缺氧适应。我们的研究结果表明，BCG生产DosR可以在标准的体外条件下培养，允许评估该菌株作为潜伏期特异性疫苗候选者。(C)2010爱思唯尔有限公司保留所有权利。
In this report we demonstrated that under aerobic conditions, Mycobacterium bovis BCG expressing an hsp60-driven second copy of the hypoxia-related transcriptional regulator DosR increased 2-fold or greater the expression of 38 out of the 48 genes belonging to the DosR regulon, including the latency antigens Rv1733c, Rv2029, Rv2627, and Rv2628. Expression of DosR under these conditions slightly delayed in vitro growth, but did not promote a non-replicating state as opposed to microaerobic and hypoxic adaptation. Our results suggest BCG producing DosR can be cultured under standard in vitro conditions, allowing evaluation of this strain as a latency-specific vaccine candidate. (C) 2010 Elsevier Ltd. All rights reserved.