Comparison of cellular response in bovine intervertebral disc cells and articular chondrocytes: effects of lipopolysaccharide on proteoglycan metabolism
Comparison of cellular response in bovine intervertebral disc cells and articular chondrocytes: effects of lipopolysaccharide on proteoglycan metabolism
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DOI：
10.1007/s00441-006-0225-1
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发表时间：
2006-12-01
影响因子：
3.6
通讯作者：
Masuda, Koichi
中科院分区：
文献类型：
--
作者：
Aota, Yoichi;An, Howard S.;Masuda, Koichi
脂多糖（LPS）诱导基质降解，并显著刺激椎间盘细胞和软骨细胞产生多种细胞因子，如白细胞介素-1 β、-6和-10。我们进行了一系列的实验来比较牛椎间盘（髓核和纤维环）和牛关节软骨细胞对LPS的细胞反应。从牛椎间盘和关节软骨中分离出海藻酸盐珠细胞，在10%胎牛血清的存在下，用LPS或不加LPS进行培养。测定DNA含量、蛋白聚糖合成和降解速率。在关节软骨细胞中，LPS以剂量依赖的方式强烈抑制细胞增殖和蛋白多糖合成，并刺激蛋白多糖降解。与关节软骨细胞相比，髓核细胞有类似的反应，尽管不那么明显。然而，用LPS处理纤维环细胞对蛋白多糖的合成和降解没有显著影响。在纤维环细胞中观察到轻微但有统计学意义的细胞增殖抑制。因此，LPS抑制蛋白多糖的合成并刺激蛋白多糖在关节软骨细胞和髓核细胞中的降解。与其他两种细胞类型相比，LPS对纤维环细胞的影响较小。脂多糖对不同类型细胞的不同作用表明这些细胞之间的代谢差异，并可能进一步表明脂多糖信号通路的差异以及对外源刺激（如脂多糖）的不同敏感性。
Lipopolysaccharide (LPS) induces matrix degradation and markedly stimulates the production of several cytokines, i.e., interleukin-1 beta, -6, and -10, by disc cells and chondrocytes. We performed a series of experiments to compare cellular responses of cells from the bovine intervertebral disc (nucleus pulposus and annulus fibrosus) and from bovine articular cartilage to LPS. Alginate beads containing cells isolated from bovine intervertebral discs and articular cartilage were cultured with or without LPS in the presence of 10% fetal bovine serum. The DNA content and the rate of proteoglycan synthesis and degradation were determined. In articular chondrocytes, LPS strongly suppressed cell proliferation and proteoglycan synthesis in a dose-dependent manner and stimulated proteoglycan degradation. Compared with articular chondrocytes, nucleus pulposus cells responded in a similar, although less pronounced manner. However, treatment of annulus fibrosus cells with LPS showed no significant effects on proteoglycan synthesis or degradation. A slight, but statistically significant, inhibition of cell proliferation was observed at high concentrations of LPS in annulus fibrosus cells. Thus, LPS suppressed proteoglycan synthesis and stimulated proteoglycan degradation by articular chondrocytes and nucleus pulposus cells. The effects of LPS on annulus fibrosus cells were minor compared with those on the other two cell types. The dissimilar effects of LPS on the various cell types suggest metabolic differences between these cells and may further indicate a divergence in pathways of LPS signaling and a differential sensitivity to exogenous stimuli such as LPS.