Regeneration of goldfish retina: rod precursors are a likely source of regenerated cells.
Regeneration of goldfish retina: rod precursors are a likely source of regenerated cells.
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金鱼视网膜的再生：视杆细胞前体是再生细胞的可能来源。
DOI：
10.1002/neu.480190504
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Rivlin,PK
中科院分区：
文献类型：
--
作者：
Raymond,PA;Reifler,MJ;Rivlin,PK
本研究描述了金鱼幼鱼神经视网膜的再生。通过眼内注射哇巴因破坏视网膜，这是Wolburg及其同事介绍的一种技术（Maier和Wolburg，1979; Kurz‐Isler和Wolburg，1982）。我们证实了他们的观察，即毒素产生的损伤水平是分级的，因为视网膜内层的神经元优先被破坏，只有在受影响更严重的视网膜中，外核层的细胞（即，感光细胞）受损。在注射哇巴因后约2周开始可以看到视网膜再生的证据。与以前的研究（Maier和Wolburg，1979）相反，我们发现再生只发生在那些光感受器被破坏的视网膜中。在外核层幸免的情况下，即使在6个月后，也没有发生内层的再生。胸苷放射自显影被用来记录新的视网膜神经元的再生，并显示杆前体，像其他分裂细胞，没有被破坏的蛋白酶，但相反，显示出增加的有丝分裂活动。再生没有进行均匀，但开始在神经源性病灶分散在整个视网膜。这些病灶由分裂的神经上皮样细胞簇组成。这些证据与这些细胞来源于视杆细胞前体的假设是一致的。这些结果表明，杆前体是能够更广泛的发展命运比他们通常表达。
This study describes regeneration of the neural retina in juvenile goldfish. The retina was destroyed with an intraocular injection of ouabain, a technique introduced by Wolburg and colleagues (Maier and Wolburg, 1979; Kurz‐Isler and Wolburg, 1982). We confirmed their observation that the level of damage produced by the toxin was graded, in that neurons in the inner retinal layers were preferentially destroyed, and only in the more severely affected retinas were cells in the outer nuclear layer (i.e., photoreceptor cells) damaged. Evidence of retinal regeneration could be seen beginning about 2 weeks after the injection of ouabain. In contrast to previous studies (Maier and Wolburg, 1979), we found that regeneration took place only in those retinas in which photoreceptors had been destroyed. In cases in which the outer nuclear layer was spared, no regeneration of inner layers occurred, even after 6 months. Thymidine autoradiography was used to document the regeneration of new retinal neurons and to show that rod precursors, like other dividing cells, were not destroyed by the oubain, but in contrast showed an increased mitotic activity. Regeneration did not proceed uniformly, but was initiated at neurogenic foci scattered across the retina. These foci consisted of clusters of dividing neuroepithelial‐like cells. The evidence is consistent with the proposal that these cells were derived from rod precursors. These results imply that rod precursors are capable of a wider range of developmental fates than they normally express.
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钠钾三磷酸腺苷酶：药理学、生理学和生化方面。
影响因子：
21.1
作者：
A. Schwartz;G. Lindenmayer;J. Allen
通讯作者：
J. Allen
影响因子：
--
作者：
T. Nork;I. Wallow;S. Sramek;G. Anderson
通讯作者：
T. Nork;I. Wallow;S. Sramek;G. Anderson
影响因子：
2.7
作者：
P. Raymond;P. Rivlin
通讯作者：
P. Raymond;P. Rivlin
Na,K 腺苷三磷酸酶在大鼠视网膜中的免疫细胞化学定位。
DOI：
10.1177/32.2.6319483
发表时间：
1984
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Stahl,WL;Baskin,DG
通讯作者：
Baskin,DG
哇巴因和刀豆球蛋白 A 在胚胎神经视网膜细胞培养物中刺激 DNA 合成。
DOI：
10.1016/0045-6039(76)90004-x
发表时间：
1976
期刊：
Cell differentiation
影响因子：
--
作者：
P. Kaplowitz;A. Moscona
通讯作者：
A. Moscona