ACUTE STRESSORS STIMULATE ION SECRETION AND INCREASE EPITHELIAL PERMEABILITY IN RAT INTESTINE
ACUTE STRESSORS STIMULATE ION SECRETION AND INCREASE EPITHELIAL PERMEABILITY IN RAT INTESTINE
复制标题
DOI：
10.1152/ajpgi.1994.267.5.g794
复制
发表时间：
1994-11-01
影响因子：
4.5
通讯作者：
PERDUE, MH
中科院分区：
文献类型：
--
作者：
SAUNDERS, PR;KOSECKA, U;PERDUE, MH
急性应激刺激大鼠肠离子分泌，增加上皮通透性。点。[j] .中国生物医学工程。肝脏生理学杂志，30(3):794- 799,1994。wistarkyoto大鼠受到4小时的约束应激（RS）或冷约束应激（CRS），并在Ussing室中检测空肠组织与非应激对照大鼠组织的运输功能变化。基线短路电流（I-sc）在RS组（相似50%）和CRS组（100%）的组织中显著升高。Cl-的取代消除了这种异常，表明应激刺激了Cl-的分泌。肠神经的跨壁电刺激引起所有组织中I-sc的短暂增加。这种反应在CRS组织中的程度明显低于对照大鼠；然而，上皮分泌的能力，以响应外源性刺激与亚酚或血管活性肠多肽未受损，暗示神经变化。RS和CRS大鼠空肠组织导度均高于对照组。惰性放射性标记探针、[H-3]甘露醇和cr -51标记的EDTA的通量显著增加，证实了应激大鼠肠上皮通透性的增加。未观察到结构变化。我们认为急性应激源对肠上皮生理、刺激离子分泌和降低屏障功能有深远的影响。
Acute stressors stimulate ion secretion and increase epithelial permeability in rat intestine. Am. J. Physiol. 267 (Gastrointest. Liver Physiol. 30): G794-G799, 1994.-WistarKyoto rats were subjected to 4 h restraint stress (RS) or cold restraint stress (CRS), and jejunal tissues were examined in Ussing chambers for alterations in transport functions compared with tissues from unstressed control rats. The baseline short-circuit current (I-sc) was significantly elevated in tissues from RS (similar to 50%) and CRS (100%). Substitution of Cl- eliminated the abnormality, suggesting that stress stimulates Cl- secretion. Electrical transmural stimulation of enteric nerves caused a transient increase in I-sc in all tissues. The magnitude of this response was significantly less in tissues from CRS than from control rats; however, the ability of the epithelium to secrete in response to exogenous stimulation with bethanechol or vasoactive intestinal polypeptide was unimpaired, implicating a neural change. Tissue conductance was higher in jejunum from RS and CRS rats than from controls. Increased intestinal epithelial permeability in stressed rats was confirmed by significantly greater fluxes of the inert radiolabeled probes, [H-3]mannitol and Cr-51-labeled EDTA. No structural changes were observed. We conclude that acute stressors have profound effects on intestinal epithelial physiology, stimulating ion secretion and reducing barrier function.