High expression of COL10A1 is associated with poor prognosis in colorectal cancer.
High expression of COL10A1 is associated with poor prognosis in colorectal cancer.
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COL10A1高表达与结直肠癌不良预后相关
DOI：
10.2147/ott.s160196
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发表时间：
2018
影响因子：
4
通讯作者：
Liu H
中科院分区：
文献类型：
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作者：
Huang H;Li T;Ye G;Zhao L;Zhang Z;Mo D;Wang Y;Zhang C;Deng H;Li G;Liu H
背景X型胶原α 1链（COL10A1）是胶原蛋白家族的一员，在多种人类肿瘤中有高表达，但其具体功能和分子机制尚不清楚。目的研究COL10A1在大肠癌组织和细胞中的表达，探讨其在大肠癌中的生物学功能和作用机制。材料与方法采用免疫组化、实时定量聚合酶链反应（QPCR）和Western blot方法检测大肠癌组织中COL10A1的表达水平。结果大肠癌组织中COL10A1的表达明显高于正常组织。生物学功能实验表明，COL10A1的过表达增强了CRC细胞的增殖、迁移和侵袭，而COL10A1的敲低抑制了体内肿瘤的发生。Western blot结果显示，COL10A1能促进上皮细胞向间质细胞转化（EMT）。组织芯片分析显示COL10A1的过表达与结直肠癌的不良预后有关。结论COL10A1在大肠癌组织中异常高表达，并参与大肠癌的发生发展和EMT的发生。此外，我们证明了COL10A1的高水平表达是结直肠癌患者预后和总生存率的独立危险因素。提示COL10A1有可能成为肿瘤治疗的新靶点。
Background High expression of collagen type X alpha 1 chain (COL10A1), a member of the collagen family, had been observed in various human cancers, but the detailed function and molecular mechanism of COL10A1 were largely unclear. Aim The aim of this study was to investigate the expression of COL10A1 in colorectal cancer (CRC) tissues and cells and to reveal its biological function and mechanism in CRC. Materials and methods Immunohistochemistry (IHC), real-time quantitative polymerase chain reaction (QPCR) and Western blot experiments were used to determine the clinical relevance between expression levels of COL10A1 and CRC. Results Compared with normal tissues, COL10A1 expression was significantly higher in CRC tissues. Biological functional experiments showed that overexpression of COL10A1 enhanced proliferation, migration, and invasion of CRC cells, and knockdown of COL10A1 inhibited tumorigenesis in vivo. Western blot assays showed that COL10A1 promoted the process of epithelial–mesenchymal transition (EMT). The overexpression of COL10A1 was associated with adverse prognosis in CRC by tissue microarray (TMA) analysis. Conclusion Our findings had provided evidences to support the fact that COL10A1 was abnormally up-expressed in CRC and involved in the progression of CRC and the process of EMT. Furthermore, we demonstrated that the high-level expression of COL10A1 was an independent risk factor of prognosis and overall survival in CRC patients. These suggested that COL10A1 might be a new potential target for cancer therapy in the future.
DOI：
10.1297/cpe.24.33
发表时间：
2015-01
期刊：
Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology
影响因子：
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作者：
Hasegawa K;Higuchi Y;Yamashita M;Tanaka H
通讯作者：
Tanaka H