A simple label-free rhodamine 6G SERS probe for quantitative analysis of trace As3+ in an aptamer-nanosol
A simple label-free rhodamine 6G SERS probe for quantitative analysis of trace As3+ in an aptamer-nanosol
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一种简单的无标记罗丹明 6G SERS 探针，用于定量分析适体纳米溶胶中的痕量 As3
DOI：
10.1039/c4ra04416a
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发表时间：
2014
期刊：
影响因子：
3.9
通讯作者：
Jiang Zhiliang
中科院分区：
文献类型：
--
作者：
Ye Lingling;Wen Guiqing;Dong Jinchao;Luo Yanghe;Liu Qingye;Liang Aihui;Jiang Zhiliang
以核酸适体（ssDNA）修饰金纳米粒子（NGs），制备As 3+的NGssDNA探针。在含有50 mmol L−1 NaCl的pH 8.0 HEPES缓冲溶液中，吸附在NGssDNA溶胶基底上的罗丹明6 G（Rh 6 G）分子在1358 cm−1处显示出强的表面增强拉曼散射峰（Sers）。加入As 3+后，Rh 6 G与NGssDNA探针反应形成稳定的As-ssDNA复合物，并释放NGs，NGs作为底物聚集到NGs聚集体（NGA）上，其中Rh 6 G Sers活性很弱。随着As 3+浓度的增加，由于形成更多的NGA，Sers峰在1358 cm-1处降低。降低的Sers强度与As 3+浓度在0.288-23.04 ng mL−1范围内呈线性关系，检测限为0.1 ng mL−1。
Gold nanoparticles (NGs) were modified by the aptamer (ssDNA) to prepare a NGssDNA probe for As3+. In pH 8.0 HEPES buffer solution containing 50 mmol L−1 NaCl, rhodamine 6G (Rh6G) molecules adsorbed on the NGssDNA sol substrate exhibited a strong surface-enhanced Raman scattering peak (SERS) at 1358 cm−1. Upon addition of As3+, it reacts with the NGssDNA probe to form a stable As–ssDNA complex and release NGs that were aggregated to the NG aggregates (NGAs) as a substrate, in which Rh6G SERS activity is very weak. With the increase of As3+ concentration, the SERS peak decreased at 1358 cm−1 due to more NGAs forming. The decreased SERS intensity responds linearly with the concentration of As3+ over 0.288–23.04 ng mL−1, with a detection limit of 0.1 ng mL−1.