Sequence and expression of a baculovirus protein with antigenic similarity to telokin.
Sequence and expression of a baculovirus protein with antigenic similarity to telokin.
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与 telokin 具有抗原相似性的杆状病毒蛋白的序列和表达。
DOI：
10.1099/0022-1317-75-7-1807
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
GuerrieroJr,V
中科院分区：
文献类型：
--
作者：
Raynes,DA;Hartshorne,DJ;GuerrieroJr,V
一种来自杆状病毒感染细胞的蛋白质与抗平滑肌蛋白telokin的抗体发生反应。由于这种不寻常的相似性，蛋白质，称为telokin样蛋白-20（TLP 20），分离和表征。其Mron变性聚丙烯酰胺凝胶电泳温度为28 K，蛋白质中β结构比例较高。分离TLP 20的cDNA并测序。3′端非编码区含有与其他两个杆状病毒基因5′端高度同源的区域。5′端非编码区含有多个杆状病毒调控元件。令人惊讶的是，衍生的氨基酸序列显示与telokin没有同源性。将该cDNA克隆到细菌表达载体中，表达的蛋白比天然蛋白的Mr略低，但与telokin抗体有交叉反应。本文报道了一种新的杆状病毒蛋白的表征，该蛋白与平滑肌蛋白telokin具有一些抗原相似性。
A protein from baculovirus-infected cells reacted with an antibody against the smooth muscle protein telokin. Because of this unusual similarity, the protein, termed telokin-like protein-20 (TLP20), was isolated and characterized. ItsMron denaturing polyacrylamide gels was 28K and the protein contained a high proportion ofβstructure. A cDNA for TLP20 was isolated and sequenced. The 3′ non-coding sequence contained a region of high identity with the 5′ end of two other baculovirus genes. The 5′ non-coding region contains several baculovirus regulatory elements. Surprisingly, the derived amino acid sequence showed no homologies to telokin. The cDNA was cloned into a bacterial expression vector and the subsequently expressed protein had a slightly lowerMrthan the native protein, but cross-reacted with telokin antibody. This paper reports the characterization of a new baculovirus protein that shares some antigenic similarities to the smooth muscle protein telokin.