Tetrahydrocannabinolic acid synthase, the enzyme controlling marijuana psychoactivity, is secreted into the storage cavity of the glandular trichomes
Tetrahydrocannabinolic acid synthase, the enzyme controlling marijuana psychoactivity, is secreted into the storage cavity of the glandular trichomes
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DOI：
10.1093/pcp/pci166
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发表时间：
2005-09-01
影响因子：
4.9
通讯作者：
Shoyama, Y
中科院分区：
文献类型：
--
作者：
Sirikantaramas, S;Taura, F;Shoyama, Y
四氢大麻酚酸 (THCA) 合酶是负责产生四氢大麻酚 (THC) 的酶，四氢大麻酚是大麻 (Cannabis sativa L.) 的精神活性成分。基于以下观察，我们在此建议 THCA 在腺毛的储存腔中生物合成。 （i）通过逆转录-PCR（RT-PCR）分析证实了THCA合酶在腺毛的分泌细胞中的唯一表达。 (ii)在储存腔内容物中检测到THCA合酶活性。 (iii)表达与绿色荧光蛋白融合的THCA合酶的转基因烟草在对应于储存腔的毛状体头部中显示出荧光。这些结果还表明，腺毛的分泌细胞不仅分泌代谢物，还分泌生物合成酶。
Tetrahydrocannabinolic acid (THCA) synthase is the enzyme responsible for the production of tetrahydrocannabinol (THC), the psychoactive component of marijuana (Cannabis sativa L.). We suggest herein that THCA is biosynthesized in the storage cavity of the glandular trichomes based on the following observations. (i) The exclusive expression of THCA synthase was confirmed in the secretory cells of glandular trichomes by reverse transcription-PCR (RT-PCR) analysis. (ii) THCA synthase activity was detected in the storage cavity content. (iii) Transgenic tobacco expressing THCA synthase fused to green fluorescent protein showed fluorescence in the trichome head corresponding to the storage cavity. These results also showed that secretory cells of the glandular trichomes secrete not only metabolites but also biosynthetic enzyme.