Catalyst-proximity protein chemical labelling on affinity beads targeting endogenous lectins
Catalyst-proximity protein chemical labelling on affinity beads targeting endogenous lectins
复制标题
靶向内源凝集素的亲和珠上的催化剂邻近蛋白化学标记
DOI：
10.1039/c9cc05231c
复制
发表时间：
2019
影响因子：
4.9
通讯作者：
Nakamura Hiroyuki
中科院分区：
文献类型：
--
作者：
Tsushima Michihiko;Sato Shinichi;Niwa Tatsuya;Taguchi Hideki;Nakamura Hiroyuki
用乳糖和Ru/dcbpy络合物功能化制备了磁性亲和小球。利用催化剂亲和标记剂MaurA，实现了乳糖结合蛋白在小球上的高选择性催化标记。结合乳糖的细胞内源性凝集素(Galectin-1和Galectin-3)的首次无偏鉴定是通过亲和珠上的化学标记实现的。
Magnetic affinity beads functionalized with lactose and ruthenium/dcbpy complexes were developed. Using MAUra, a catalyst-proximity labelling reagent, the catalytic labeling of lactose-binding proteins was achieved with high selectivity on the beads. The first unbiased identification of cellular endogenous lectins bound to lactose (galectin-1 and galectin-3) was achieved with chemical labelling on the affinity beads.