Cellular and subcellular distribution of D‐aspartate oxidase in human and rat brain
Cellular and subcellular distribution of D‐aspartate oxidase in human and rat brain
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D-天冬氨酸氧化酶在人和大鼠脑中的细胞和亚细胞分布
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
H. Fahimi
中科院分区：
文献类型：
--
作者：
K. Zaar;H. Köst;A. Schad;A. Völkl;E. Baumgart;H. Fahimi
D -天冬氨酸在大脑和内分泌腺中大量存在，并被认为参与神经传递和神经分泌（Wolosker et al. [2000] Neuroscience 100:183-189）。D -天冬氨酸氧化酶是唯一已知的代谢D -天冬氨酸的酶，可以调节其在大脑不同区域的水平。我们用免疫组织化学、原位杂交和免疫电镜检查了该酶及其mRNA在人和大鼠脑中的细胞和亚细胞分布。D -天冬氨酸氧化酶蛋白和mRNA普遍存在。该蛋白呈颗粒状，尤其是在神经元内，在星形胶质细胞和少突胶质细胞中较少。没有观察到突触关联的证据。而在大多数阳性神经元之间只观察到逐渐的差异，在下丘脑室旁核中，发现酶含量高的神经元与其他未标记的神经元相邻。D -天冬氨酸氧化酶的cDNA克隆证实了蛋白质导入过氧化物酶体的固有靶向信号序列。免疫电镜显示该蛋白定位于单膜结合细胞器，明显是过氧化物酶体。[j]中华神经医学杂志，2002。©2002 Wiley‐Liss, Inc。
The unusual amino acid D‐aspartate is present in significant amounts in brain and endocrine glands and is supposed to be involved in neurotransmission and neurosecretion (Wolosker et al. [ 2000 ] Neuroscience 100:183–189). D‐aspartate oxidase is the only enzyme known to metabolize D‐aspartate and could regulate its level in different regions of the brain. We examined the cellular and subcellular distribution of this enzyme and its mRNA in human and rat brain by immunohistochemistry, in situ hybridization, and immunoelectron microscopy. D‐aspartate oxidase protein and mRNA are ubiquitous. The protein shows a granular pattern, particularly within neurons and to a significantly lesser extent in astrocytes and oligodendrocytes. No evidence for a synaptic association was observed. Whereas between most positive neurons only gradual differences were observed, in the hypothalamic paraventricular nucleus, neurons with high enzyme content were found next to others with no labeling. cDNA cloning of D‐aspartate oxidase corroborates an inherent targeting signal sequence for protein import into peroxisomes. Immunoelectron microscopy showed that the protein is localized in single membrane‐bound organelles, apparently peroxisomes. J. Comp. Neurol. 450:272–282, 2002. © 2002 Wiley‐Liss, Inc.
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来自牛肾的 D-天冬氨酸氧化酶。
DOI：
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发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Negri,A;Massey,V;WilliamsJr,CH
通讯作者：
WilliamsJr,CH
D-天冬氨酸对大鼠大细胞神经分泌系统的调节：哺乳动物中天然存在的游离 D-氨基酸的生物学作用的证据。
DOI：
10.1677/joe.0.1670247
发表时间：
2000
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Wang,H;Wolosker,H;Pevsner,J;Snyder,SH;Selkoe,DJ
通讯作者：
Selkoe,DJ
影响因子：
4.8
作者：
G. D'aniello;A. Tolino;A. D’Aniello;F. Errico;G. Fisher;M. M. Di Fiore-M.
通讯作者：
G. D'aniello;A. Tolino;A. D’Aniello;F. Errico;G. Fisher;M. M. Di Fiore-M.
DOI：
10.1073/pnas.96.2.721
发表时间：
1999-01-19
影响因子：
11.1
作者：
Wolosker, H;Sheth, KN;Snyder, SH
通讯作者：
Snyder, SH
DOI：
10.1073/pnas.96.23.13409
发表时间：
1999-11-09
影响因子：
11.1
作者：
Wolosker, H;Blackshaw, S;Snyder, SH
通讯作者：
Snyder, SH