Transcriptome analysis of the aphid bacteriocyte, the symbiotic host cell that harbors an endocellular mutualistic bacterium, Buchnera
Transcriptome analysis of the aphid bacteriocyte, the symbiotic host cell that harbors an endocellular mutualistic bacterium, Buchnera
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DOI：
10.1073/pnas.0409034102
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发表时间：
2005-04-12
影响因子：
11.1
通讯作者：
Fukatsu, T
中科院分区：
文献类型：
--
作者：
Nakabachi, A;Shigenobu, S;Fukatsu, T
蚜虫拥有细菌细胞，这种细胞被专门分化为含有布氏菌属的强制性互惠细菌，这种细菌失去了许多对常见细菌功能至关重要的基因。为了了解宿主在维持共生关系中的作用，我们从豌豆蚜虫Acyrthosiphon pisum中分离出细菌细胞，并利用EST分析和实时定量RT-PCR对宿主转录组进行了研究。许多基因在细菌细胞中特异性高表达，包括(t)氨基酸代谢基因，包括那些生物合成布赫内拉不能产生的氨基酸的基因，以及那些利用布赫内拉能合成的氨基酸的基因；(h)与运输相关的基因，包括线粒体转运蛋白基因和编码Rab（一种调节囊泡运输的G蛋白）的基因；（iii）降解细菌细胞壁的推定溶菌酶基因。i的显著上调清楚地表明，细菌细胞参与了寄主蚜虫和布氏虫之间的氨基酸交换，这是共生系统中关键的代谢过程。ii和iii的显著高表达揭示了共生系统中宿主- buchnera相互作用的未知方面。
Aphids possess bacteriocytes, cells specifically differentiated to harbor obligatory mutualistic bacteria of the genus Buchnera, which have lost many genes that are essential for common bacterial functions. To understand the host's role in maintaining the symbiotic relationship, bacteriocytes were isolated from the pea aphid, Acyrthosiphon pisum, and the host transcriptome was investigated by using EST analysis and real-time quantitative RT-PCR. A number of genes were highly expressed specifically in the bacteriocyte, including (t) genes for amino acid metabolism, including those for biosynthesis of amino acids that Buchnera cannot produce, and those for utilization of amino acids that Buchnera can synthesize; (h) genes related to transport, including genes for mitochondrial transporters and a gene encoding Rab, a G protein that regulates vesicular transport; and (iii) genes for putative lysozymes that degrade bacterial cell walls. Significant up-regulation of i clearly indicated that the bacteriocyte is involved in the exchange of amino acids between the host aphid and Buchnera, the key metabolic process in the symbiotic system. Conspicuously high expression of ii and iii shed light on previously unknown aspects of the host-Buchnera interactions in the symbiotic system.