Protective efficacy of multivalent replication-abortive vaccine strains in horses against African horse sickness virus challenge.
Protective efficacy of multivalent replication-abortive vaccine strains in horses against African horse sickness virus challenge.
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DOI：
10.1016/j.vaccine.2017.06.023
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发表时间：
2017-07-24
期刊：
影响因子：
5.5
通讯作者：
Roy P
中科院分区：
文献类型：
--
作者：
Lulla V;Losada A;Lecollinet S;Kerviel A;Lilin T;Sailleau C;Beck C;Zientara S;Roy P
AHSV是一种新出现的虫媒病毒。受感染马匹的死亡率通常高达90%，新一代疫苗是利用遗传学方法制造的。这些疫苗复制失败，但免疫原性高。单价和多价疫苗对小马都有保护作用。非洲马瘟病毒（African horse sickness virus，AHSV）是呼肠孤病毒科（Reoviridae）的一种环状病毒。到目前为止，已经描述了九种不同的血清型。AHSV由库蠓属（Culicoides spp.）对马科动物造成了很高的死亡率，特别是马的死亡率，对经济造成了相当大的影响。为了开发安全的减毒疫苗，我们先前建立了一种有效的反向遗传学（RG）系统，以产生所有9种血清型的进入复制-流产（ECRA）病毒株，并证明了这些毒株在I型干扰素受体（IFNAR）敲除小鼠中的疫苗潜力。在这里，我们评估了这些ECRA病毒在AHSV天然宿主中的保护效力。在小马中检测了一种单血清型（ECRA.A4）疫苗和一种多价混合疫苗（ECRA.A1/4/6/8），随后用强毒AHSV 4攻毒。与对照动物相比，所有接种疫苗的小马都受到保护，没有出现严重的AHS临床症状。此外，多价混合物接种的小马产生针对混合物中存在的所有血清型的中和抗体，并且在试验期间出生的马驹是健康的并且没有病毒血症。这些结果验证了这些ECRA菌株作为新一代AHSV疫苗的适用性。
AHSV is an emerging insect-borne virus. The mortality rate of infected horses is often up to 90% New generation of vaccines are made using reveres genetics. These vaccines are replication abortive but highly immunogenic. Both monovalent and multivalent vaccines are protective in ponies. African horse sickness virus (AHSV) is an orbivirus, a member of the Reoviridae family. Nine different serotypes have been described so far. AHSV is vectored by Culicoides spp. to equids, causing high mortality, particularly in horses, with considerable economic impacts. For development of a safe attenuated vaccine, we previously established an efficient reverse genetics (RG) system to generate Entry Competent Replication-Abortive (ECRA) virus strains, for all nine serotypes and demonstrated the vaccine potential of these strains in type I interferon receptor (IFNAR)-knockout mice. Here, we evaluated the protective efficacies of these ECRA viruses in AHSV natural hosts. One monoserotype (ECRA.A4) vaccine and one multivalent cocktail (ECRA.A1/4/6/8) vaccine were tested in ponies and subsequently challenged with a virulent AHSV4. In contrast to control animals, all vaccinated ponies were protected and did not develop severe clinical symptoms of AHS. Furthermore, the multivalent cocktail vaccinated ponies produced neutralizing antibodies against all serotypes present in the cocktail, and a foal born during the trial was healthy and had no viremia. These results validate the suitability of these ECRA strains as a new generation of vaccines for AHSV.
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影响因子：
5.4
作者：
Celma CC;Stewart M;Wernike K;Eschbaumer M;Gonzalez-Molleda L;Breard E;Schulz C;Hoffmann B;Haegeman A;De Clercq K;Zientara S;van Rijn PA;Beer M;Roy P
通讯作者：
Roy P
影响因子：
3.7
作者：
Bachanek-Bankowska K;Maan S;Castillo-Olivares J;Manning NM;Maan NS;Potgieter AC;Di Nardo A;Sutton G;Batten C;Mertens PP
通讯作者：
Mertens PP
影响因子：
5.5
作者：
Alberca, Berta;Bachanek-Bankowska, Katarzyna;Cabana, Marta;Calvo-Pinilla, Eva;Viaplana, Elisenda;Frost, Lorraine;Gubbins, Simon;Urniza, Alicia;Mertens, Peter;Castillo-Olivares, Javier
通讯作者：
Castillo-Olivares, Javier
影响因子：
3.8
作者：
Roy, P;Bishop, DHL;Erasmus, B
通讯作者：
Erasmus, B
影响因子：
5.4
作者：
Boyce, Mark;Celma, Cristina C. P.;Roy, Polly
通讯作者：
Roy, Polly