Diagnosis of Norovirus outbreaks by commercial ELISA or RT-PCR
Diagnosis of Norovirus outbreaks by commercial ELISA or RT-PCR
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DOI：
10.1016/j.jviromet.2006.06.024
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发表时间：
2006-11-01
影响因子：
3.1
通讯作者：
Koopmans, Marion P. G.
中科院分区：
文献类型：
--
作者：
de Bruin, Erwin;Duizer, Erwin;Koopmans, Marion P. G.
对IDEIA诺瓦克样病毒（Dakocytomation Ltd., Ely, uk）和Ridascreen诺瓦克样病毒酶免疫测定（R-Biopharm AG, Darmstadt, Germany）的急性胃肠炎暴发诊断进行了评估。来自23次暴发的158份粪便样本，包括确认的轮状病毒和星状病毒暴发，用于确定两种ELISA试剂盒相对于RT-PCR方案的敏感性和特异性，随后采用Southern blot杂交。另一组由6个不同的基因组菌株、12个基因组II菌株和1个基因组IV菌株组成，用于确定试验范围。与RT-PCR相比，Dako试剂盒和Ridascreen试剂盒的敏感性分别为38%和36%，特异性分别为96%和88%。2株基因I和1株基因11未被Dako试剂盒检测到，5株基因I和5株基因11未被Ridascreen试剂盒检测到。两种ELISA试剂盒的灵敏度和Ridascreen的检测范围都令人失望。然而，ELISA试剂盒可用于初步筛查，前提是ELISA阴性的疫情将通过RT-PCR方法重新检测。(c) 2006 Elsevier B.V.版权所有
The IDEIA Norwalk-like virus (Dakocytomation Ltd., Ely, United Kingdom) and the Ridascreen Norwalk-like virus enzyme immunoassay (R-Biopharm AG, Darmstadt, Germany), were evaluated for the diagnosis of outbreaks of acute gastroenteritis.A panel of 158 fecal samples from 23 outbreaks, including confirmed rotavirus and astrovirus outbreaks, was used to determine the sensitivity and specificity of both ELISA kits relative to an RT-PCR protocol that was followed by Southern blot hybridization. Another panel consisted of 6 different genogroup [strains, 12 genogroup II strains and 1 genogroup IV strain and was used to determine the scope of the tests.Compared to the RT-PCR, sensitivities of 38% and 36% and specificities of 96% and 88% were found for the Dako kit and the Ridascreen kit, respectively. Two genogroup I strains, and one genogroup 11 strain were not detected by the Dako kit, while five genogroup I and five genogroup 11 strains were not detected by the Ridascreen kit.The sensitivity of both ELISA kits, and the scope of the Ridascreen are considered disappointing. However, the ELISA kits can be useful for a preliminary screening, provided that ELISA negative outbreaks will be re-tested by RT-PCR methods. (c) 2006 Elsevier B.V. All rights reserved.