The 5' ends of the transforming gene of Fujinami sarcoma virus and of the cellular proto-fps gene are not colinear.
The 5' ends of the transforming gene of Fujinami sarcoma virus and of the cellular proto-fps gene are not colinear.
复制标题
藤波肉瘤病毒的转化基因和细胞原fps基因的5末端不共线。
DOI：
10.1016/0042-6822(84)90414-8
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发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
Duesberg,PH
中科院分区：
文献类型：
--
作者：
Seeburg,PH;Lee,WH;Nunn,MF;Duesberg,PH
测定了富士那美肉瘤病毒(FSV)细胞原fps区的500个核苷酸的序列，该区包括与Fujinami肉瘤病毒fps序列重叠的5‘端。结果发现，对于不包括Aug翻译起始密码子的44个密码子，原fps区的潜在阅读框架持续超过与病毒fps重叠的5‘边界。已经确定了一个超过91个密码子的潜在的原fps上游外显子和一个潜在的在offps/proto-fpsoverap处的剪接受体。结论：原FP与病毒FP之间的重叠边界不代表原FP编码区的5‘端。原fps基因起始于与Fsv不同的上游外显子(S)，或至少51个密码子与Fsv重叠边界下游。此外，原fps的5‘编码区不同于口蹄疫病毒(Δgas-fps)杂合转化基因，在没有逆转录病毒基因衍生序列的情况下。
The sequence of 500 nucleotides of a cellular proto-fpsregion that includes the 5′ boundary of overlap with thefpssequence of Fujinami sarcoma virus (FSV) was determined. It was found that the potential reading frame of the proto-fpsregion continues over the 5′ boundary of overlap with viralfpsfor 44 codons which do not include an AUG translation initiation codon. A potential upstream exon of proto-fpsof over 91 codons and a potential splice acceptor at the site offps/proto-fpsoverlap have been identified. It is concluded that the boundary of overlap between proto-fpsand viralfpscannot represent the 5′ end of the coding region of proto-fps. The proto-fpsgene starts either in the upstream exon(s) not shared with FSV or at least 51 codons downstream of the boundary of overlap with FSV. In addition, the 5′ coding region of proto-fpsdiffers from the (Δgas-fps) hybrid transforming gene of FSV in the absence of the retroviralgaggene-derived sequence.