Clinical, immunological, and immunogenetic aspects of autoantibody production against Ro/SSA, La/SSB and their linear epitopes in primary Sjogren's syndrome (pSS): a European multicentre study
Clinical, immunological, and immunogenetic aspects of autoantibody production against Ro/SSA, La/SSB and their linear epitopes in primary Sjogren's syndrome (pSS): a European multicentre study
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DOI：
10.1136/ard.61.5.398
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发表时间：
2002-05-01
影响因子：
27.4
通讯作者：
Moutsopoulos, HM
中科院分区：
文献类型：
--
作者：
Tzioufas, AG;Wassmuth, R;Moutsopoulos, HM
Objectives: To investigate the clinical and immunogenetic aspects of antibody formation against Ro/SSA and La/SSB as well as their linear B cell epitopes in patients with primary Sjogren's syndrome (pSS) from different European countries.Patients and methods: Ninety patients with pSS from six European centres were studied.所有患者的血清样本均在对照实验室中通过 RNA 沉淀测定检测抗 Ro/SSA 和抗 La/SSB 自身抗体，以及针对先前报道的 Ro 60 kDa（p169-190aa 和 p211-232aa）和 La/SSB（p147-154aa、p291-302aa、p291-302aa）的 B 细胞线性表位的自身抗体。 p301-318aa 和 p349-364aa）。来自 88 名患者的 DNA 用于确定 HLA-DRB1、-DQA1 和 -DQB1 基因型。对 88 名进行了基因分型并测试了抗肽抗体的患者进行了结果分析。结果：以较低频率（范围 10-37%）检测到 Ro 60 kDa B 细胞表位的抗体。 In contrast, B cell epitopes of La/SSB were detected frequently (range 58-86%) among the anti-La/SSB positive sera. La/SSB 表位 p349-364aa 的自身抗体与较长的病程呈显着正相关 (p
Objectives: To investigate the clinical and immunogenetic aspects of antibody formation against Ro/SSA and La/SSB as well as their linear B cell epitopes in patients with primary Sjogren's syndrome (pSS) from different European countries.Patients and methods: Ninety patients with pSS from six European centres were studied. Serum samples from all patients were tested in a control laboratory for anti-Ro/SSA and anti-La/SSB autoantibodies by RNA precipitation assay and autoantibodies to the previously reported B cell linear epitopes of Ro 60 kDa (p169-190aa and p211-232aa) and La/SSB (p147-154aa, p291-302aa, p301-318aa, and p349-364aa). DNA from 88 patients was used for the determination of HLA-DRB1, -DQA1, and -DQB1 genotypes. Analysis of the results was performed in the 88 patients who were genotyped and tested also for antipeptide antibodies.Results: Antibodies to B cell epitopes of Ro 60 kDa were detected at a low frequency (range 10-37%). In contrast, B cell epitopes of La/SSB were detected frequently (range 58-86%) among the anti-La/SSB positive sera. Autoantibodies to the La/SSB epitope, p349-364aa, were significantly positively associated with longer disease duration (p