Interleukin-4 enhances interferon-gamma synthesis but inhibits development of interferon-gamma-producing cells.
Interleukin-4 enhances interferon-gamma synthesis but inhibits development of interferon-gamma-producing cells.
复制标题
Interleukin-4 增强干扰素 γ 合成，但抑制干扰素 γ 产生细胞的发育。
作者：
A. Noble;D. Kemeny
白细胞介素-4（IL-4）对干扰素-γ（IFN-γ）的许多活性具有拮抗作用，并且据报道，除了抑制1型辅助性T细胞（Th 1）的发育外，还直接阻断人淋巴细胞中IFN-γ的合成。然而，IL-4转基因小鼠产生增加量的IFN-γ以及IL-4。我们比较了大鼠IL-4调节IFN-γ分泌的能力，在短期培养的脾细胞与其对T淋巴细胞分化成IFN-γ产生，或Th 1型，细胞的影响。使用多种促分裂原和卵清蛋白抗原刺激正常大鼠脾细胞，有或没有IL-4，持续12-24小时，并通过酶联免疫吸附测定（ELISA）测量上清液中IFN-γ的水平。结果表明，当用植物血凝素（PHA）或刀豆球蛋白A（ConA）刺激正常大鼠脾细胞时，IL-4在12-24小时后增强IFN-γ的分泌。当用明矾沉淀的卵清蛋白免疫10天前的动物的脾细胞在体外用卵清蛋白刺激时，这种增强也很明显，并且似乎主要通过CD+ T细胞介导。相反，当脾细胞最大限度地刺激佛波醇肉豆蔻酸酯乙酸酯（PMA）和离子霉素，除了IL-4的IFN-γ分泌量没有影响。当脾细胞在IL-4存在下用Con A刺激4天，并用PMA和离子霉素再刺激时，IFN-γ分泌被大大抑制。我们的研究结果表明，IL-4发挥不同的影响IFN-γ分泌和IFN-γ产生淋巴细胞的发展。
Interleukin-4 (IL-4) is antagonistic for many of the activities of interferon-gamma (IFN-gamma) and, as well as suppressing the development of T-helper type-1 (Th1) cells, has been reported to block directly the synthesis of IFN-gamma in human lymphocytes. However, IL-4 transgenic mice produce increased amounts of IFN-gamma as well as IL-4. We have compared the ability of rat IL-4 to regulate IFN-gamma secretion in short-term cultures of spleen cells with its effect on the differentiation of T lymphocytes into IFN-gamma-producing, or Th1-type, cells. Normal rat spleen cells were stimulated using a variety of mitogens and ovalbumin antigen, with or without IL-4, for 12-24 hr and the levels of IFN-gamma in the supernatants measured by enzyme-linked immunosorbent assay (ELISA). The results show that when normal rat splenocytes were stimulated with phytohaemagglutinin (PHA) or concavalin A (Con A), IL-4 enhanced secretion of IFN-gamma after 12-24 hr. This enhancement was also apparent when splenocytes from animals immunized 10 days previously with alum-precipitated ovalbumin were stimulated with ovalbumin in vitro, and appeared to be mediated primarily via CD+ T cells. In contrast, when spleen cells were maximally stimulated with phorbol myristate acetate (PMA) and ionomycin, addition of IL-4 had no effect on the amount of IFN-gamma secreted. When splenocytes were stimulated with Con A for 4 days in the presence of IL-4, and restimulated with PMA and ionomycin, IFN-gamma secretion was greatly suppressed. Our results indicate that IL-4 exerts differential effects on IFN-gamma secretion and on the development of IFN-gamma-producing lymphocytes.
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影响因子：
4.4
作者：
T. Gajewski;F. Fitch
通讯作者：
T. Gajewski;F. Fitch
影响因子：
4.4
作者：
S. Swain;A. Weinberg;M. English;G. Huston
通讯作者：
S. Swain;A. Weinberg;M. English;G. Huston
细胞色素 c 特异性产生 IL-4 的 T 细胞的调节和发育。
DOI：
--
发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Betz,M;Fox,BS
通讯作者：
Fox,BS
IL-4 抑制由 IFN-γ 介导的人培养单核细胞的 H2O2 产生和抗利什曼尼能力。
DOI：
--
发表时间：
1989
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lehn,M;Weiser,WY;Engelhorn,S;Gillis,S;Remold,HG
通讯作者：
Remold,HG