Evidence for expression of the C3d receptor of Candida albicans in vitro and in vivo obtained by immunofluorescence and immunoelectron microscopy.
Evidence for expression of the C3d receptor of Candida albicans in vitro and in vivo obtained by immunofluorescence and immunoelectron microscopy.
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通过免疫荧光和免疫电子显微镜获得的白色念珠菌 C3d 受体在体外和体内表达的证据。
DOI：
10.1128/iai.59.5.1832-1838.1991
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发表时间：
1991
影响因子：
3.1
通讯作者：
Cutler,JE
中科院分区：
文献类型：
--
作者：
Kanbe,T;Li,RK;Wadsworth,E;Calderone,RA;Cutler,JE
补体转化产物 C3d 与白色念珠菌细胞表面的受体结合。虽然该受体的功能仍不确定，但我们研究了它是否在小鼠感染期间表达。针对纯化受体产生的兔抗血清与免疫荧光显微镜和免疫胶体金电子显微镜结合使用，检查感染小鼠的肾组织和腹膜灌洗液中白色念珠菌体内的受体表达。抗血清的特异性通过与纯化受体（55 至 60 kDa）的反应性以及与来自完整菌丝提取物的蛋白质印迹（免疫印迹）中具有相似分子量的蛋白质的反应性来表明。免疫荧光显微镜的体外分析表明，抗血清与酵母和假菌丝形式的生物体发生反应，但与假菌丝的反应性最强。对腹腔灌洗液中的真菌细胞进行免疫胶体金电子显微镜观察，发现萌发形式的母细胞、芽管和假菌丝有强烈的染色。母细胞的染色在细胞壁最内层最重，但在细胞表面很少。相反，在整个芽管和假菌丝的细胞壁中观察到染色。在肾脏中，C3d 受体的表达主要在菌丝和假菌丝的细胞壁上发现，尽管在细胞质中观察到一些染色。这些数据支持白色念珠菌的C3d受体在体内表达。
The complement conversion product C3d binds to a receptor on the cell surface of Candida albicans. While the function of this receptor is still uncertain, we investigated whether it is expressed during a murine infection. Rabbit antiserum raised against purified receptor was used in conjunction with immunofluorescence microscopy and immunocolloidal gold electron microscopy to examine kidney tissue and peritoneal lavages from infected mice for receptor expression by C. albicans in vivo. Specificity of the antiserum was indicated by reactivity with purified receptor (55 to 60 kDa) and with a protein of similar molecular mass from whole hyphal extracts in Western blots (immunoblots). In vitro analysis by immunofluorescence microscopy showed that the antiserum reacted with both yeast and pseudohyphal forms of the organism, but reactivity was strongest with pseudohyphae. Immunocolloidal gold electron microscopy of fungal cells from peritoneal lavages revealed intense staining of mother cells of germinative forms, germ tubes, and pseudohyphae. Staining of the mother cells was heaviest at the innermost layers of the cell wall but only scant on the cell surface. In contrast, staining was observed throughout the cell walls of germ tubes and pseudohyphae. In kidney, expression of the C3d receptor was found primarily on the cell walls of hyphae and pseudohyphae, although some staining was observed in the cytoplasm. These data support that the C3d receptor of C. albicans is expressed in vivo.