Generation of a IoxP flanked Bmp4IoxP-IacZ allele marked by conditional IacZ expression
Generation of a IoxP flanked Bmp4IoxP-IacZ allele marked by conditional IacZ expression
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DOI：
10.1002/gene.10032
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发表时间：
2002-02-01
期刊：
影响因子：
1.5
通讯作者：
Hogan, BLM
中科院分区：
文献类型：
--
作者：
Kulessa, H;Hogan, BLM
为了检测Cre诱导的Bmp4基因(编码骨形态发生蛋白4的基因)的重组以及随后的β-半乳糖苷酶的表达，将BmploxP-lacZ小鼠与表达Cre重组酶的HoxB4-Cre转基因小鼠杂交。只有携带Bmp4等位基因和转基因的胚胎才显示出Z的表达。β-半乳糖苷酶活性，表明重组事件，在一个区域观察到反映重叠的Cret转基因和正常的Bmp4表达模式。这表明重组等位基因的TatlacZ表达忠实地报告了Bmp4基因正常表达区域中Bmp4蛋白编码序列的删除。随着合适的Cre转基因小鼠品系的出现，FloxedBmp4等位基因将能够分析Bmp4在器官发生和出生后的整个生命过程中的功能。
To test for Cre-induced recombination of the floxedBmp4locus (the gene encoding for bone morphogenetic protein 4) with consequent expression of β-galactosidase,BmploxP-lacZmice were crossed withHoxB4-Cretransgenic mice that express Cre recombinase in posterior mesodermal and neural tissues. Only embryos carrying the floxedBmp4allele and theCretransgene showlacZexpression. β-galactosidase activity, indicative of the recombinant event, is observed in a domain reflecting the overlap of theCretransgene and normalBmp4expression pattern. This shows tatlacZexpression from the recombined floxed allele faithfully reports excision of theBmp4protein coding sequence in the normal expression domain of theBmp4gene. As suitable Cre transgenic mouse lines become available, the floxedBmp4allele will allow analysis ofBmp4function, throughout organogenesis and postnatal life.