Scaffold-dependent differentiation of human articular chondrocytes
Scaffold-dependent differentiation of human articular chondrocytes
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DOI：
10.3892/ijmm_00000074
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发表时间：
2008-11-01
影响因子：
5.4
通讯作者：
Marlovits, Stefan
中科院分区：
文献类型：
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作者：
Schlegel, Werner;Nuernberger, Sylvia;Marlovits, Stefan
基质相关自体软骨细胞移植(MACT)是一种将自体软骨细胞种植在生物材料上的组织工程方法，用于治疗软骨缺损。本研究的目的是分析人关节软骨细胞在三种用于MACT的不同基质上的生长和分化行为。人关节软骨细胞在单层培养42天后种植在由I/III型胶原、透明质酸或明胶组成的基质上。在4周的培养时间内，每周对构建物进行分析。通过扫描和透射电子显微镜观察其形态特征。用实时荧光定量聚合酶链式反应(Real-time PCR)分析主要类型胶原的表达。I型/III型胶原基质支持与天然软骨的组织结构非常相似的分化，但细胞数量和II型胶原合成较少，分化发生在培养后期。透明质酸基质和明胶基质支持较快的分化，有大量的细胞和相对较多的II型胶原，但没有模仿天然软骨的空间组装。这些事实表明，基质的性质对去分化软骨细胞的分化行为有很大的影响。
Matrix-associated autologous chondrocyte transplantation (MACT) is a tissue-engineered approach for the treatment of cartilage defects and combines autologous chondrocytes seeded on biomaterials. The objective of the study is the analysis of growth and differentiation behaviour of human articular chondrocytes grown on three different matrices used for MACT. Human articular chondrocytes were kept in monolayer culture for 42 days and then seeded on matrices consisting of either collagen type I/III, hyaluronan, or gelatine. During the culture time of 4 weeks the constructs were analyzed weekly. Morphological criteria were studied by scanning and transmission electron microscopy. The expression of the main type collagens was analyzed by real-time PCR. The collagen type I/III matrix supported a differentiation that closely resembled the tissue organisation of native cartilage, but cell number and type II collagen synthesis were low and differentiation occurred rather late in the cultivation period. The hyaluronan matrix and the gelatine-based matrix supported a rather rapid differentiation, with a high number of cells and a relatively high amount of type II collagen, but there was no spatial assembly that mimicked native cartilage. These facts indicate that the nature of the matrix is of great influence in the differentiation behaviour of dedifferentiated chondrocytes.