Investigation of the microRNAs in safflower seed, leaf, and petal by high-throughput sequencing
Investigation of the microRNAs in safflower seed, leaf, and petal by high-throughput sequencing
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DOI：
10.1007/s00425-010-1327-2
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发表时间：
2011-03-01
期刊：
影响因子：
4.3
通讯作者：
Li, Xiaokun
中科院分区：
文献类型：
--
作者：
Li, Haiyan;Dong, Yuanyuan;Li, Xiaokun
红花（红花）是已用于生产油质蛋白的最重要的作物之一。miRNAs（microRNAs）是一类在转录后水平负调控基因表达的小分子非编码RNA，在植物生长发育和逆境应答中发挥重要作用。本研究利用高通量Illumina测序技术对红花种子、花和叶的小RNA转录组进行了全面的研究。研究发现，红花中至少有236种已知的miRNAs表达，其中100种miRNAs表达丰度较高，在多种植物中具有进化保守性。不同组织中表达丰度的比较显示，总共有116、133和128个miRNA在红花种子/叶、种子/花瓣和叶/花瓣之间以较高丰度或较低丰度显著差异表达。组织间miRNA丰度的最显著差异中的大多数是组织特异性miRNA。此外，在红花中已经鉴定出13种推定的新miRNAs。本研究中获得的小RNA转录组为进一步研究红花中已鉴定的miRNA的生理作用提供了基础。
Safflower (Carthamus tinctorius L.) is one of the most important crop plants that has been utilized for production of oleosins. miRNAs (microRNAs) are a class of small and non-coding RNAs that negatively regulate gene expression at post-transcriptional level thus playing a role in plant growth, development, and stress response. In this study, high-throughput Illumina sequencing technology has been used to comprehensively investigate the small RNA transcriptomes of safflower seed, flower, and leaf. It is found that there are at least 236 known miRNAs expressed in safflower, of which 100 miRNAs with relatively high expression abundance exhibited evolutionary conservation across multiple plants. Comparison of their expression abundance among different tissues shows that a total of 116, 133, and 128 miRNAs are significantly differentially expressed with higher abundance or lower abundance between safflower seed/leaf, seed/petal, and leaf/petal. The majority of the most significant differences in miRNA abundance between tissues are tissue-specific miRNAs. In addition, 13 putative novel miRNAs have been identified in safflower. The small RNA transcriptomes obtained in this study provide a basis for further investigation of the physiological roles of identified miRNAs in safflower.