Establishment of the Visceral Embryonic Midline Is a Dynamic Process that Requires Bilaterally Symmetric BMP Signaling
Establishment of the Visceral Embryonic Midline Is a Dynamic Process that Requires Bilaterally Symmetric BMP Signaling
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DOI：
10.1016/j.devcel.2016.05.018
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发表时间：
2016-06-20
影响因子：
11.8
通讯作者：
Schultheiss, Thomas M.
中科院分区：
文献类型：
--
作者：
Arraf, Alaa A.;Yelin, Ronit;Schultheiss, Thomas M.
脊椎动物的身体计划包含背侧和腹侧中线结构。虽然背侧中线结构已被广泛研究，但腹侧中线结构的形成以及它们如何与背侧中线对齐，是脊椎动物发育的一个基本方面，但人们知之甚少。本研究使用鸡背肠系膜 (DM) 作为模型来研究腹侧中线结构的形成。我们记录了通过上皮间质转化（EMT）和侧板体腔衬里内侧进入形成的 DM，并表明 DM 定位是一个基本的动态过程，受进入侧板两侧骨形态发生蛋白（BMP）信号相对水平的调节。这一过程的破坏会导致 DM 的错位和肺形态发生初始阶段的干扰。由于背侧中线是 BMP 拮抗剂的来源，这些结果表明了一种对齐背侧和腹侧胚胎中线的机制。
The vertebrate body plan contains both dorsal and ventral midline structures. While dorsal midline structures have been extensively studied, formation of ventral midline structures, and how they become aligned with the dorsal midline, is a fundamental aspect of vertebrate development that is poorly understood. This study uses the chick dorsal mesentery (DM) as a model for investigating the formation of ventral midline structures. We document formation of the DM by epithelial-to-mesenchymal transition (EMT) and medial ingression of the lateral plate coelomic lining and show that DM positioning is a fundamentally dynamic process regulated by relative levels of bone morphogenetic protein (BMP) signaling in the two sides of the ingressing lateral plate. Disruption of this process causes misalignment of the DM and disturbances during initial stages of lung morphogenesis. Since the dorsal midline is a source of BMP antagonists, these results suggest a mechanism for aligning the dorsal and ventral embryonic midlines.