CLONING OF THE GENE CODING FOR A SHARED HUMAN-MELANOMA ANTIGEN RECOGNIZED BY AUTOLOGOUS T-CELLS INFILTRATING INTO TUMOR
CLONING OF THE GENE CODING FOR A SHARED HUMAN-MELANOMA ANTIGEN RECOGNIZED BY AUTOLOGOUS T-CELLS INFILTRATING INTO TUMOR
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DOI：
10.1073/pnas.91.9.3515
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发表时间：
1994-04-26
影响因子：
11.1
通讯作者：
ROSENBERG, SA
中科院分区：
文献类型：
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作者：
KAWAKAMI, Y;ELIYAHU, S;ROSENBERG, SA
通过cDNA表达克隆，我们已经分离出一个基因编码的HLA-A2限制性自体和同种异体肿瘤浸润淋巴细胞（TILs）从转移性黑色素瘤患者识别的共享人类黑色素瘤抗原。通过使用瞬时和稳定表达系统，将该基因转染到不表达抗原的HLA-A2（+）细胞系中导致抗原特异性TIL的识别。该cDNA的序列揭示了一种以前未描述的推定跨膜蛋白，其表达仅限于黑色素瘤和黑素细胞系和人视网膜，但没有检测到其他新鲜或培养的正常组织或其他肿瘤组织。因此，我们已经鉴定了编码黑素细胞谱系特异性蛋白（MART-1; T细胞识别的黑色素瘤抗原1）的基因，该基因是被已确定的恶性肿瘤患者的T淋巴细胞识别的广泛共有的黑色素瘤抗原。该基因的鉴定为黑色素瘤患者的免疫疗法的发展开辟了可能性。
By cDNA expression cloning we have isolated a gene encoding a shared human melanoma antigen recognized by HLA-A2 restricted autologous and allogeneic tumor-infiltrating lymphocytes (TILs) from patients with metastatic melanoma. By using both transient and stable expression systems, transfection of this gene into non-antigen-expressing HLA-A2(+) cell lines resulted in recognition by the antigen-specific TILs. The sequence of this cDNA revealed a previously undescribed putative transmembrane protein whose expression was restricted to melanoma and melanocyte cell lines and human retina but no other fresh or cultured normal tissues tested or other tumor histologies. Thus, we have identified a gene encoding a melanocyte lineage-specific protein (MART-1; melanoma antigen recognized by T cells 1) that is a widely shared melanoma antigen recognized by the T lymphocytes of patients with established malignancy. Identification of this gene opens possibilities for the development of immunotherapies for patients with melanoma.