Breeding structure of three snow pool Aedes mosquito species in northern Colorado.
Breeding structure of three snow pool Aedes mosquito species in northern Colorado.
复制标题
科罗拉多州北部三种雪池伊蚊的繁殖结构。
DOI：
10.1046/j.1365-2540.1998.00387.x
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发表时间：
1998
期刊：
影响因子：
3.8
通讯作者：
Black4th,WC
中科院分区：
文献类型：
--
作者：
West,DF;Black4th,WC
使用 RAPD-PCR 多态性检查科罗拉多州北部三个河流流域的三种雪池伊蚊的繁殖结构。从四个雪池中收集了卡塔伊蚊 (Aedes cataphylla Dyar) 的幼虫、七个雪池 (Aedes hexodontus Dyar) 和六个雪池 (Aedes pullatus (Coquillet)) 的幼虫。对每个物种的 47-48 个 RAPD 位点进行多态性评分。为了测试距离的隔离性，根据地理距离绘制了池对之间的 F ST/(1− F ST) 值，并进行了 1000 次随机排列的 Mantel 测试。 F ST 值在 A. cataphylla 和 A. hexodontus 中与地理距离无关，但在 A. pullatus 中有些相关，表明该物种中存在距离隔离。对水池中的成对 F ST 值进行了聚类分析，其中包括 1994 年和 1995 年采样的七个水池。Bootstrap 分析表明，水池跨流域聚集，通常与地理邻近性无关。然而，人们一致支持多年来从同一池中收集的 A. cataphylla 和高海拔 A. pullatus 收集的幼虫聚集在一起。山脉似乎并不是这些物种基因流动的主要障碍。相反，成虫羽化的季节差异可能成为A. pullatus 和A. cataphylla 种群迁移的障碍。 A. hexodontus 的幼虫连续分布在河岸的草丛中，当春季径流期间这些水池被冲走时，幼虫的随机死亡可能会导致遗传漂变。
RAPD-PCR polymorphisms were used to examine breeding structure in three species of snow pool Aedes mosquitoes across three river drainages in northern Colorado. Larvae were collected from four snow pools for Aedes cataphylla Dyar, seven pools for A. hexodontus Dyar and six pools for A. pullatus (Coquillet). Polymorphisms were scored at 47–48 RAPD loci in each species. To test for isolation by distance, F ST/(1− F ST) values between pairs of pools were plotted against geographical distances and subjected to a Mantel test with 1000 random permutations. F ST values were independent of geographical distances in A. cataphylla and A. hexodontus but were somewhat correlated in A. pullatus suggesting isolation by distance in this species. A cluster analysis was performed on pair-wise F ST values among pools including seven pools that were sampled in both 1994 and 1995. Bootstrap analysis indicated that pools clustered across drainages and generally independently of geographical proximity. However, there was consistent support for clustering of larvae collected from the same pool across years in A. cataphylla and in high altitude collections of A. pullatus. Mountains do not appear to act as major barriers to gene flow in any of these species. Instead, seasonal differences in adult emergence may serve as barriers to migration among A. pullatus and A. cataphylla populations. Larvae of A. hexodontus are distributed continuously in grassy pools along the banks of rivers and genetic drift probably occurs through random larval mortality when these pools are washed out during spring run-off.