Regional localization on the human X of DNA segments cloned from flow sorted chromosomes.
Regional localization on the human X of DNA segments cloned from flow sorted chromosomes.
复制标题
从流式分选染色体克隆的 DNA 片段在人类 X 上的区域定位。
DOI：
10.1093/nar/10.5.1557
复制
发表时间：
1982
影响因子：
14.9
通讯作者：
S. Latt
中科院分区：
文献类型：
--
作者：
Louis M. Kunkel;Umadevi Tantravahi;M. Eisenhard;S. Latt
用荧光激活的方法对49，XXXXY人淋巴母细胞的染色体进行分选，以获得富含人X染色体的DNA。该DNA克隆到λ噬菌体Charon 21A中，获得一个约60,000 pfu的文库。通过两个独立的杂交分析，将不含人类高重复DNA序列的噬菌体插入片段定位到人类X的不同区域。第一种是利用比较杂交技术对包含人类X染色体全部或已知部分的啮齿动物-人类杂交细胞DNA样本进行杂交，而第二种方法是基于对X染色体或X染色体片段数目不同的人类细胞系DNA样本的杂交剂量。在测试的5个唯一序列中，有3个是X染色体特有的，分别定位于XpT导联到Xcen、Xq1导联到Xq22和Xq24导联到Xqter区域。这里提供的文库代表了高度丰富的人类X染色体特异DNA序列的来源。
Fluorescence activated sorting of chromosomes from 49,XXXXY human lymphoblasts has been used to obtain DNA enriched for the human X. This DNA was cloned in lambda phage Charon 21A to obtain a library of approximately 60,000 pfu. Phage inserts free of human highly repeated DNA sequences are localized to different regions of the human X by two independent hybridization analyses. The first utilized comparative hybridization to rodent-human hybrid cell DNA samples containing all or known portions of the human X, while the second was based on hybridization dosage to DNA samples from human cell lines differing in the number of X chromosomes or X chromosome segments. Of five unique sequence inserts tested, three were X chromosome specific and were localized to regions Xpter leads to Xcen, Xql leads to Xq22 and Xq24 leads to Xqter, respectively. The library presented here represents a highly enriched source of human X chromosome-specific DNA sequences.