Advanced approaches for elucidating structures of large RNAs using NMR spectroscopy and complementary methods
Advanced approaches for elucidating structures of large RNAs using NMR spectroscopy and complementary methods
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DOI：
10.1016/j.ymeth.2020.01.009
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发表时间：
2020-11-01
期刊：
影响因子：
4.8
通讯作者：
Keane, Sarah C.
中科院分区：
文献类型：
--
作者：
Kotar, Anita;Foley, Hannah N.;Keane, Sarah C.
核磁共振波谱是一项关键技术，它极大地促进了我们对RNA结构和动力学的理解。然而，通过核磁共振光谱法确定大RNA结构仍然是一个重大的技术挑战。在这篇综述中，我们强调了促进大RNA的NMR研究的进展，包括样品制备方法，同位素标记策略和数据采集。此外，我们审查的混合方法，已在大RNA的结构测定。
NMR spectroscopy is a key technique that has significantly advanced our understanding of RNA structure and dynamics. However, determination of large RNA structures by NMR spectroscopy remains a significant technical challenge. In this review, we highlight advances that facilitate NMR studies of large RNAs, including methods for sample preparation, isotope labeling strategies, and data acquisition. In addition, we review hybrid approaches that have been instrumental in the structure determination of large RNAs.