HDA6 is required for jasmonate response, senescence and flowering in Arabidopsis
HDA6 is required for jasmonate response, senescence and flowering in Arabidopsis
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DOI：
10.1093/jxb/erm300
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发表时间：
2008-02-01
影响因子：
6.9
通讯作者：
Chaikam, Vijaya
中科院分区：
文献类型：
--
作者：
Wu, Keqiang;Zhang, Lin;Chaikam, Vijaya
翻译后修饰组蛋白的阳离子，包括乙酰化，在调节染色质结构和基因活性的动态变化中起关键作用。组蛋白乙酰化通过组蛋白乙酰转移酶和去乙酰化酶的作用来调节。HDA 6是拟南芥中的RPD 3型组蛋白脱乙酰酶。与野生型相比，拟南芥HDA 6突变体、axe 1 -5和HDA 6 RNA干扰（HDA 6-RNAi）植物显示出更高水平的乙酰化H3，表明HDA 6影响组蛋白乙酰化水平。在axe 1 -5和HDA 6-RNAi植物中，茉莉酸响应基因PDF1.2、VSP 2、JIN 1和ERF 1的表达下调。此外，与野生型相比，axe 1 -5和HDA 6-RNAi植物显示出增加的叶寿命。衰老相关基因SAG 12和SEN 4的表达在axe 1 -5和HDA 6-RNAi植物中下调。此外，axe 1 -5和HDA 6-RNAi植物显示开花较晚。FLC的表达在axe 1 -5和HDA 6-RNAi植物中上调和过度乙酰化，表明HDA 6需要使FLC染色质脱乙酰化，从而抑制其表达。我们的研究结果表明，HDA 6参与茉莉酸响应，衰老，开花拟南芥。
Post-translational modi. cations of histones, including acetylation, play a key role in modulating dynamic changes in chromatin structure and gene activity. Histone acetylation is modulated through the action of histone acetyltransferases and deacetylases. HDA6 is a RPD3-type histone deacetylase in Arabidopsis. The Arabidopsis HDA6 mutant, axe1-5, and HDA6 RNA-interfering ( HDA6-RNAi) plants displayed higher levels of acetylated H3 compared with wild-type, suggesting that HDA6 affects histone acetylation levels globally. The expression of the jasmonate responsive genes, PDF1.2, VSP2, JIN1, and ERF1, was down-regulated in axe1-5 and HDA6-RNAi plants. Furthermore, axe1-5 and HDA6-RNAi plants displayed increased leaf longevity compared with the wild type. The expression of the senescence-associated genes, SAG12 and SEN4, was down-regulated in the axe1-5 and HDA6-RNAi plants. In addition, axe1-5 and HDA6-RNAi plants displayed late-flowering. The expression of FLC was up-regulated and hyperacetylated in axe1-5 and HDA6-RNAi plants, suggesting that HDA6 is required to deacetylate FLC chromatin and thereby repress its expression. Our results suggest that HDA6 is involved in jasmonate response, senescence, and flowering in Arabidopsis.