Changes in growth and photosynthesis of mixotrophic Ochromonas sp. in response to different concentrations of glucose
Changes in growth and photosynthesis of mixotrophic Ochromonas sp. in response to different concentrations of glucose
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DOI：
10.1007/s10811-016-0832-5
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发表时间：
2016-03
影响因子：
3.3
通讯作者：
Lu Zhang;Bangping Li;Zhongqiu Wu;L. Gu;Zhou Yang
中科院分区：
文献类型：
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作者：
Lu Zhang;Bangping Li;Zhongqiu Wu;L. Gu;Zhou Yang
Ochromonassp。根据其在田间的普遍营养策略，菊花科植物具有不同的营养水平。能利用无机碳和有机碳进行光自养、混合养和异养生长。目的探讨外源有机碳对油菜种群生长和光合作用的影响。，我们培养了dochromonassp。在不同葡萄糖浓度（0、50、100和150 mg L−1）的培养基中，采用两种处理模式（开始时单次添加葡萄糖和每12 h重复添加一次的分批喂料模式），持续15天。结果表明：(1)与光自养生长相比，添加葡萄糖显著提高了ochromonassp的生长速度和携带能力。以浓度依赖的方式；(2)在指数生长阶段，光合作用也随着葡萄糖的添加而增加；(3)与单次添加相比，重复添加葡萄糖能提高生物量和光合性能；(4)随着葡萄糖的消耗，种群达到一个平稳阶段，光合参数略有下降，但仍保持在一个相对稳定的水平，这与光自养生长相似。结果表明，与纯光自养相比，混合营养生长可在更短的时间内获得更高的种群生长率和最终生物量，特别是在饲料分批培养中，这将是一种高效的大规模培养鞭毛藻的方式。
Ochromonassp. (Chrysophyceae) occupies different trophic levels depending on its prevalent nutritional strategy in the field.Ochromonassp. can utilize inorganic and organic carbon to grow photoautotrophically, mixotrophically, and heterotrophically. To examine how exogenous organic carbon influences population growth and photosynthesis inOchromonassp., we culturedOchromonassp. in media with different glucose concentrations (0, 50, 100, and 150 mg L−1) and two treatment modes (single addition of glucose at the beginning and fed-batch mode with repeated addition every 12 h) for 15 days. Results showed that (1) compared to photoautotrophic growth, adding glucose significantly enhanced the growth rate and carrying capacity ofOchromonassp. in a concentration-dependent manner; (2) photosynthesis also increased with glucose addition during the exponential growth stage; (3) repeated glucose addition caused higher biomass and photosynthetic performances than single addition, and (4) with depletion of glucose and the population reaching a stationary stage, photosynthetic parameters decreased slightly but remained at a relatively stable level, which was similar to that of photoautotrophic growth. We conclude that in comparison with pure photoautotrophy, mixotrophic growth can result in higher population growth rate and final biomass in a shorter time, especially in fed-batch cultures, indicating that this would be a highly efficient way for mass cultivation of the flagellateOchromonas.