A niche maintaining germ line stem cells in the Drosophila ovary
A niche maintaining germ line stem cells in the Drosophila ovary
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DOI：
10.1126/science.290.5490.328
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发表时间：
2000-10-13
期刊：
影响因子：
56.9
通讯作者：
Spradling, AC
中科院分区：
文献类型：
--
作者：
Xie, T;Spradling, AC
基质细胞被认为产生控制干细胞行为的特定调节微环境或“小生境”。表征干细胞在体内的小生境仍然是一个重要的目标，一直难以实现。果蝇卵巢的每个卵巢管含有大约两个维持卵母细胞产生的生殖系干细胞。在这里，我们表明，包括三种细胞类型的前卵巢体细胞作为一个生殖系干细胞龛。由于正常或诱导分化而丢失的生殖系干细胞被有效地替代，并且重新填充小生境的能力增加了体内卵巢管的功能寿命。我们的研究涉及体细胞类型之一，帽细胞，作为一个关键的生态位组成部分。
Stromal cells are thought to generate specific regulatory microenviroments or "niches" that control stem cell behavior. Characterizing stem cell niches in vivo remains an important goal that has been difficult to achieve. The individual ovarioles of the Drosophila ovary each contain about two germ line stem cells that maintain oocyte production. Here we show that anterior ovariolar somatic cells comprising three cell types act as a germ line stem cell niche. Germ line stem cells Lost by normal or induced differentiation are efficiently replaced, and the ability to repopulate the niche increases the functional lifetime of ovarioles in vivo. Our studies implicate one of the somatic cell types, the cap cells, as a key niche component.