EzrA Contributes to the Regulation of Cell Size in Staphylococcus aureus
EzrA Contributes to the Regulation of Cell Size in Staphylococcus aureus
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DOI：
10.1371/journal.pone.0027542
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发表时间：
2011-11-14
期刊：
影响因子：
3.7
通讯作者：
Pinho, Mariana G.
中科院分区：
文献类型：
--
作者：
Jorge, Ana M.;Hoiczyk, Egbert;Pinho, Mariana G.
Ezra是枯草芽孢杆菌中FtsZ的负调控因子，参与细胞生长和分裂的协调以及细胞伸长-分裂周期的控制。我们现在已经研究了枯草杆菌Ezra蛋白的金黄色葡萄球菌同源物的作用，并表明它对细胞活力不是必需的。与野生型菌株相比，Ezra条件突变体和零突变体的平均细胞大小总体上都有所增加。在较大的Ezra突变体金黄色葡萄球菌细胞中，细胞分裂蛋白FtsZ和合成青霉素结合蛋白(PBPs)的细胞壁没有正确定位。这表明，可能存在一个最大细胞直径，该直径允许形成一个Z环，该Z环能够招募分裂体的其他成分并驱动胞质分裂。我们认为Ezra在金黄色葡萄球菌中的主要作用是维持细胞大小的动态平衡。
EzrA is a negative regulator of FtsZ in Bacillus subtilis, involved in the coordination between cell growth and cell division and in the control of the cell elongation-division cycle. We have now studied the role of the Staphylococcus aureus homologue of the B. subtilis EzrA protein and shown that it is not essential for cell viability. EzrA conditional and null mutants have an overall increase of the average cell size, compared to wild type strains. In the larger ezrA mutant S. aureus cells, cell division protein FtsZ and the cell wall synthesizing Penicillin Binding Proteins (PBPs) are not properly localized. This suggests that there may be a maximum cell diameter that allows formation of a Z-ring capable of recruiting the other components of the divisome and of driving cytokinesis. We propose that the major role of EzrA in S. aureus is in cell size homeostasis.