Evaluation of a cell-permeable barrier for guided tissue regeneration combined with demineralized bone matrix.
Evaluation of a cell-permeable barrier for guided tissue regeneration combined with demineralized bone matrix.
复制标题
评估细胞渗透屏障与脱矿骨基质相结合的引导组织再生。
DOI：
10.1046/j.0905-7161.2003.00966.x
复制
发表时间：
2003
影响因子：
4.3
通讯作者：
T. Karring
中科院分区：
文献类型：
--
作者：
N. Mardas;L. Kostopoulos;A. Stavropoulos;T. Karring
目的
AIM
To evaluate whether bone formation by guided tissue regeneration (GTR) and demineralized bone matrix (DBM) can be enhanced by the use of a cell-permeable Teflon barrier allowing the penetration of undifferentiated mesenchymal cells from the surrounding soft tissues.
MATERIAL AND METHODS
DBM was produced from the long bones of rats, and its bone-inductive properties were tested in three rats prior to the study by intramuscular implantation. Thirty, 4-month-old, male albino rats of the Wistar strain were used. Following surgical exposure of the mandibular ramus, a cell-permeable Teflon capsule, loosely packed with DBM, was placed with its opening facing the lateral surface of the ramus (test side). At the contralateral side, serving as control, a non-perforated (cell-occlusive) Teflon capsule, loosely packed with the same amount of DBM, was placed. After healing periods of 30, 60, and 120 days, groups of 10 animals were killed, and 40-70 microm thick undecalcified sections of the capsules were produced.
RESULTS
Computer-assisted planimetric measurements on the histological sections disclosed similar amounts of newly formed bone in both test and control capsules. After 4 months, the new bone in the control capsules occupied 45.0% of the cross-sectional area of the capsule, while it was 50.5% in the test capsules. This difference was not statistically significant (P<0.05).
CONCLUSION
Similar amounts of bone formed in cell-permeable and cell-occlusive capsules grafted with DBM, suggesting that invasion of undifferentiated mesenchymal cells from the surrounding soft tissues into the barrier-protected area is unnecessary for bone formation with GTR.