Hydrophilic nanofibrous structure of polylactide; fabrication and cell affinity
Hydrophilic nanofibrous structure of polylactide; fabrication and cell affinity
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DOI：
10.1002/jbm.a.30695
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发表时间：
2006-08-01
影响因子：
4.9
通讯作者：
Kim, Hak Yong
中科院分区：
文献类型：
--
作者：
Bhattarai, Shanta Raj;Bhattarai, Narayan;Kim, Hak Yong
支架的微观结构和结构沿着表面化学对生物活性（细胞分布、增殖和分化）产生深远的影响。由于其生物活性，组织工程支架被广泛设计。本研究的目的是通过静电纺丝技术开发亲水性聚乳酸（PLLA）聚合物纳米纤维结构的非织造垫的形式，并进一步评估成纤维细胞NIH 3 T3细胞增殖，形态，和细胞-基质相互作用。通过在静电纺丝液中加入少量低分子量聚乙二醇（PEG），提高了PLLA纤维的亲水性。制备了4种不同比例（100/0、80/20、70/30和50/50）的PLLA/PEG静电纺丝基质，并对其孔结构、拉伸性能、接触角和水解降解性能进行了研究。并通过扫描电镜和荧光肌动蛋白染色观察细胞与基质的相互作用和细胞形态。结果表明，PLLA/PEG（80/20）电纺纤维网的纤维直径在540-850 nm之间，大部分孔径小于100 μ m，拉伸强度为8 MPa，伸长率为150%，孔隙率大于90%，亲水性改善，水解降解缓慢，有利于NIH 3 T3成纤维细胞的生物活性。基于这些结果，多孔电纺基质中PLLA和PEG的正确组成（即，PLLA/PEG（80/20）是一种比其它PLLA/PEG组合物以及疏水性PLLA更好的组织工程应用材料。(c)2006 Wiley Periodicals，Inc.
Microstructure and architecture of the scaffolds along with the surface chemistry exert profound effect on biological activity (cell distribution, proliferation, and differentiation). For the biological activity, scaffolds in tissue engineering have been widely designed. The objective of this study was to develop hydrophilic nanofibrous structure of polylactides (PLLA) polymer in the form of nonwoven mat by electrospinning technique, and further evaluate the fibroblast NIH3T3 cell proliferation, morphology, and cell-matrix interaction. Hydrophilicity of the PLLA fibers was improved by adding small fraction of low molecular weight polyethylene glycol (PEG) into the electrospinning solution. Four different ratio types (100/0, 80/20, 70/30, and 50/50) of PLLA/PEG electrospun matrices were fabricated, and the pore characteristics, tensile properties, contact angle, and hydrolytic degradation were observed. Furthermore, scanning electron microscope (SEM) and fluorescence actin staining images were used for micro-observation of cell-matrix interaction and cell morphology. It was found that the electrospun mat of PLLA/PEG (80/20), composed of fibers with diameters in the range 540-850 nm, majority of pore diameter less than 100 mu m, tensile strength 8 MPa, elongation 150%, porosity more than 90%, and improved hydrophilicity with slow hydrolytic degradation, is favorable for biological activity of NIH3T3 fibroblast cell. Based on these results, the correct composition of PLLA and PEG in the porous electrospun matrix (i.e., PLLA/PEG (80/20)) will be a better candidate rather than other compositions of PLLA/PEG as well as hydrophobic PLLA for application in tissue engineering. (c) 2006 Wiley Periodicals, Inc.