Mucoepidermoid carcinoma of upper aerodigestive tract: clinicopathologic study of 78 cases with immunohistochemical analysis of dicer expression
Mucoepidermoid carcinoma of upper aerodigestive tract: clinicopathologic study of 78 cases with immunohistochemical analysis of dicer expression
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DOI：
10.1007/s00428-007-0574-5
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发表时间：
2008-06-01
期刊：
影响因子：
3.5
通讯作者：
Seethala, R. R.
中科院分区：
文献类型：
--
作者：
Chiosea, S. I.;Barnes, E. L.;Seethala, R. R.
MicroRNA (miR) 是一种小型非编码 RNA，预计可调节高达 30% 的蛋白质编码基因。 miR 成熟需要功能性 microRNA 机制，包括 Dicer 蛋白。我们回顾了我们在粘液表皮样癌 (MEC) 方面的经验，并描述了 Dicer 表达的预后价值。通过免疫组织化学评估 78 个 MEC 中 Dicer 的表达。相对于内部对照：大排泄管/横纹管或正常鳞状上皮（粘膜）的基底/副基底层，对 Dicer 表达进行半定量评分。然后将 Dicer 评分与临床和病理参数相关联。 Dicer 过度表达和/或表达不足在高级别 MEC (83%) 中比在低/中级别 MEC (35%; p= 0.002) 中更常见，在 III/IV 期 MEC (80%) 中比在 I/II 期 MEC (41%;p= 0.04) 中更常见。 Dicer 表达异常与高级别和晚期相关，可作为 MEC 疾病特异性生存 (DSS) 不良的单变量预测因子。多变量分析显示，年龄和分期是 DSS 不良的独立预测因素。侵袭性 MEC 中 Dicer 的异常免疫表达表明 miR 和 miR 机制在肿瘤进展中的作用。
MicroRNAs (miR) are small noncoding RNAs that are predicted to regulate up to 30% of protein-encoding genes. miR maturation requires functional microRNA machinery, including the Dicer protein. We review our experience with mucoepidermoid carcinoma (MEC) and characterize the prognostic value of Dicer expression. Expression of Dicer was assessed in 78 MEC by immunohistochemistry. Dicer expression was scored semiquantitatively and relative to the internal controls: large excretory/striated ducts or basal/parabasal layers of normal squamous epithelium ( mucosa). Dicer scores were then correlated with clinical and pathologic parameters. Dicer over-and/or under-expression were more commonly seen in high-grade MEC (83%) than in low/intermediate grade MEC (35%; p= 0.002) and in stage III/IV MEC (80%) than in stage I/II MEC (41%;p= 0.04). Abnormal Dicer expression correlates with high-grade and advanced stage, acting as a univariate predictor of poor disease-specific survival (DSS) in MEC. Age and stage were independent predictors of poor DSS on multivariate analysis. Abnormal immunoexpression of Dicer in aggressive MEC suggests a role for miR and miR machinery in tumor progression.