Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment.
Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment.
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DOI：
10.1084/jem.159.4.1149
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发表时间：
1984-04-01
影响因子：
15.3
通讯作者：
Hensley, L L
中科院分区：
文献类型：
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作者：
Haynes, B F;Scearce, R M;Lobach, D F;Hensley, L L
使用鼠单克隆抗体TE-4和TE-7提出了对人胸腺基质，我们确定了两个不同的和相互排斥的胸腺微环境组件：胸腺内分泌上皮（TE-4+）和中胚层来源的纤维基质（TE-7+）。TE-4反应性上皮不与抗体TE-7反应，含有胸腺素α 1和角蛋白，并表达胸腺内分泌上皮的其他已知标记物（A2 B5和p19）。此外，TE-4+胸腺上皮细胞强烈表达I类（HLA-A、-B和-C）和II类（Ia样）主要组织相容性复合体（MHC）抗原。相反，TE-7+胸腺纤维基质不与抗体TE-4反应，不含胸腺素α 1也不含角蛋白，并且不表达胸腺内分泌上皮标记物A2 B5和p19。TE-7+胸腺基质细胞弱表达I类抗原，不表达II类MHC抗原。TE-4+和TE-7+胸腺微环境隔间是可识别的胸腺从7周妊娠到成年生活。妊娠7周时，TE-7+间质围绕着一个圆柱形的TE-4+，A2 B5+胸腺上皮原基。胎龄10 ~ 15周时，TE-7+胸腺间质包绕早期胸腺小叶。15周胎儿妊娠，抗体TE- 4定义的包膜下皮质和髓质区的内分泌胸腺上皮，而抗体TE-7结合小叶间纤维隔膜，血管和胸腺纤维囊。虽然对内分泌胸腺上皮具有特异性，但抗体TE-4与皮肤、扁桃体、结膜和食管上部的鳞状上皮基底层反应。
Using murine monoclonal antibodies TE-4 and TE-7 raised against human thymic stroma, we identified two distinct and mutually exclusive thymic microenvironment components: the thymic endocrine epithelium (TE-4+) and mesodermal-derived fibrous stroma (TE-7+). TE-4-reactive epithelium did not react with antibody TE-7, contained thymosin alpha 1 and keratin, and expressed other known markers of thymic endocrine epithelium (A2B5 and p19). Moreover, TE-4+ thymic epithelial cells strongly expressed class I (HLA-A, -B and -C) and class II (Ia-like) major histocompatibility complex (MHC) antigens. In contrast, TE-7+ thymic fibrous stroma did not react with antibody TE-4, did not contain thymosin alpha 1 nor keratin, and did not express the thymic endocrine epithelium markers A2B5 and p19. TE-7+ thymic stromal cells weakly expressed class I and did not express class II MHC antigens. Both TE-4+ and TE-7+ thymic microenvironment compartments were identifiable in thymus from 7 wk gestation through adult life. At 7 wk fetal gestation, TE-7+ stroma surrounded a cylindrical TE-4+, A2B5+ thymic epithelial rudiment. Between 10 and 15 wk fetal gestation, TE-7+ thymic stroma surrounded early thymic lobules. By 15 wk fetal gestation, antibody TE- 4 defined subcapsular cortical and medullary zones of endocrine thymic epithelium, while antibody TE-7 bound to interlobular fibrous septae, vessels, and thymic fibrous capsule. While otherwise specific for endocrine thymic epithelium, antibody TE-4 reacted with the basal layer of squamous epithelium in skin, tonsil, conjunctiva, and upper esophagus.