Phylogenetic and pathogenic analyses of two virulent Newcastle disease viruses isolated from Crested Ibis (Nipponia nippon) in China
Phylogenetic and pathogenic analyses of two virulent Newcastle disease viruses isolated from Crested Ibis (Nipponia nippon) in China
复制标题
中国朱鹮(Nipponia nippon)分离的两种强效新城疫病毒的系统发育和病原学分析
DOI：
10.1007/s11262-013-0881-7
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发表时间：
2013-06-01
期刊：
影响因子：
1.6
通讯作者：
Yang, Zengqi
中科院分区：
文献类型：
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作者：
Chen, Shengli;Hao, Huafang;Yang, Zengqi
朱鹮是世界上最濒危的鸟类之一，仅在中国中部的陕西省发现，目前已在日本佐渡岛重新引入。从患病朱鹮身上采集了两种新城疫病毒（NDV）分离株，并对其致病和系统发育特征进行了研究。结果显示，它们具有强毒力，脑内致病性指数为1.46-1.83，平均死亡时间为54.4-84.4小时。它们在 F 蛋白裂解位点具有相同的有毒基序 112-R-R-Q-K-R-F-117。系统发育分析显示，两种分离株均与 II 类 NDV 聚类，其中一种为基因型 VIId，另一种为新基因型（暂定为 VIi）。这两个分离株与2006年至2010年同一地区家禽群中分离的毒株具有高度同源性。我们首先从朱鹮中分离和鉴定了NDV分离株，其中一株表现出新的遗传特征，并与同一地区的鸽子和鸵鸟分离株形成了新的亚基因型。这些数据对于进一步开展新城疫流行病学研究和朱鹮的保护具有重要意义。
The crested ibis is one of the most endangered birds in the world, found only in Shaanxi Province in Central China, and it has been reintroduced in Sadogashima in Japan. Two Newcastle disease virus (NDV) isolates were collected from sick crested ibises, and their pathogenic and phylogenetic characteristics were investigated. The results showed that they are virulent, with intracerebral pathogenicity indices of 1.46–1.83 and a mean time of death of 54.4–84.4 h. They shared the same virulent motif112-R-R-Q-K-R-F-117at the F protein cleavage site. The phylogenetic analysis revealed that both isolates were clustered with class II NDVs, with one in genotype VIId and another in a novel genotype (provisionally designated as VIi). The two isolates shared high homology with the strains isolated from poultry flocks in the same region from 2006 to 2010. We first isolated and characterised the NDV isolates from crested ibises, one of which showed new genetic characteristics and formed a new subgenotype with isolates from pigeons and ostriches in the same area. These data are useful for further epidemiological studies on NDV and the protection of crested ibises.