Characteristics of the binding features of Nelin with F-actin and screening Nelin interactive proteins
Characteristics of the binding features of Nelin with F-actin and screening Nelin interactive proteins
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DOI：
10.1360/04wc0196
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发表时间：
2004-12-01
影响因子：
--
通讯作者：
Qu, S
中科院分区：
文献类型：
--
作者：
Wang, Z;Meng, XM;Qu, S
采用F-actin体外共沉淀法和共定位法研究了人新基因nelin的心脏特异性表达蛋白nelin与F-actin的相互作用。结果表明，nelin是一种新的f -肌动蛋白结合蛋白，可与f -肌动蛋白在细胞质中共聚落。利用酵母双杂交筛选技术，从人心脏cDNA文库中鉴定出3个新的nelin结合蛋白，分别为丝素C亚型、titin N2B亚型和α -胰蛋白酶抑制剂重链前体（ITIH）。用共免疫沉淀法证实了丝素C与亚麻素的结合活性。丝蛋白C通过其C端区与nelin结合。结果表明，nelin是一种细胞骨架相关蛋白，并作为膜-细胞骨架相关蛋白参与局灶黏附的形成。
The interaction of nelin, a cardiac-specific expressed protein of human novel gene nelin, with F-actin was studied by both F-actin cosedimentation in vitro and colocalization assays. The results showed that nelin is a new F-actin binding protein and is colocolized with F-actin in cytoplasm of cells. Three new nelin binding proteins, filamin C subtype, titin N2B subtype and inter-alpha trypsin inhibitor heavy chain precursor (ITIH), were identified from human heart cDNA library using yeast two-hybrid screening. The binding activity of filamin C with nelin was confirmed by coimmunoprecipitation. Filamin C binds to nelin through its C-terminal region. It is indicated that nelin is a cytoskeleton associated protein and acts as a membrane-cytoskeleton associated protein involved in the formation of focal adhesions.