Mutants and isolates of Metarhizium anisopliae are diverse in their relationships between conidial pigmentation and stress tolerance
Mutants and isolates of Metarhizium anisopliae are diverse in their relationships between conidial pigmentation and stress tolerance
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DOI：
10.1016/j.jip.2006.06.008
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发表时间：
2006-11-01
影响因子：
3.4
通讯作者：
Roberts, Donald W.
中科院分区：
文献类型：
--
作者：
Rangel, Drauzio E. N.;Butler, Michael J.;Roberts, Donald W.
分生孢子色素沉着在几种真菌中参与对热和UV辐射的保护。在这项研究中，我们比较了17个颜色突变体的野生型ARSEF 23和13个颜色突变体的野生型ARSEF 2575金龟子绿僵菌变种的耐受性。绿僵菌对湿热和UV-B或模拟太阳辐射的敏感性。每个突变体的胁迫耐受性与其野生型亲本进行了比较，并与迄今为止我们测试的最耐热和紫外线的野生型绿僵菌M。小孢acridum（ARSEF 324）.每种分离物或突变体的颜色用PANTONE颜色标准书[Jumeeman，L.，赫伯特，L.，1990. The PANTONE（R）Book of Color：超过1000种颜色标准：设计、时尚、家具和装饰的颜色基础和指南。和更多. Harry N.艾布拉姆斯公司，出版社，纽约]。此外，提取每个突变体或野生型的色素，并将提取物的UV吸收率与这些分离物的胁迫耐受性进行比较;但未检测到相关性。一般来说，ARSEF 23的颜色突变体比它们的亲本野生型更不耐紫外线。分生孢子色素对ARSEF 23及其突变体的UV-B和模拟太阳辐射耐受性有重要影响，而对ARSEF 2575及其突变体的影响较小。ARSEF 2575颜色突变体的紫外耐受性比ARSEF 23的变化小，即使在两组突变体中发生非常相似的颜色。当ARSEF 23的颜色突变体恢复到野生型颜色时，它们恢复了野生型水平的UV耐受性。野生型ARSEF 23和ARSEF 2575分生孢子的UV-B和UV-A暴露结果表明，它们对UV-A具有相同的耐受性，但对UV-B的反应不同。对于耐热性，一些突变体比它们的野生型亲本更耐热。因此，野生型分离株较深的色素沉着对耐热保护并不重要。(c)2006爱思唯尔公司All rights reserved.
Conidial pigmentation is involved in protection against heat and UV radiation in several fungal species. In this study, we compare the tolerance of 17 color mutants of wild-type ARSEF 23 plus 13 color mutants of wild-type ARSEF 2575 of Metarhizium anisopliae var. anisopliae to wet-heat and UV-B or simulated-solar radiation. The stress tolerance of each mutant was compared with that of its wild-type parent, and with the most thermo- and UV-tolerant wild-type Metarhizium we have tested to date, M. anisopliae var. acridum (ARSEF 324). The color of each isolate or mutant was identified with the PANTONE Color Standard book [Eiseman, L., Herbert, L., 1990. The PANTONE((R)) Book of Color: over 1000 color standards: color basics and guidelines for design, fashion, furnishing... and more. Harry N. Abrams, Inc., Publishers, New York]. In addition, the pigments of each mutant or wild-type were extracted and the UV absorbances of the extracts compared to the stress tolerance of those isolates; but no relationships were detected. Color mutants of ARSEF 23, in general, were less UV tolerant than their parent wild-type. With ARSEF 23 and its mutants, conidial pigmentation was important to conidial tolerance to UV-B and simulated-solar radiation; but color had less impact on ARSEF 2575 and its mutants. The ARSEF 2575 color mutants were less variable in UV tolerance than those of ARSEF 23, even though very similar colors occurred in the two groups of mutants. When color mutants of ARSEF 23 reverted to wild-type color they recovered wild-type levels of UV tolerance. Results of UV-B and UV-A exposures of wild-types ARSEF 23 and ARSEF 2575 conidia indicated that they are equally tolerant of UV-A, but differ in UV-B-response. For thermo tolerance, several mutants were more heat tolerant than their wild-type parents. Accordingly, darker pigmentation of wild-type isolates was not important to protection against heat. (c) 2006 Elsevier Inc. All rights reserved.