Determination of para-Chloroaniline and Reactive Oxygen Species in Chlorhexidine and Chlorhexidine Associated with Calcium Hydroxide
Determination of para-Chloroaniline and Reactive Oxygen Species in Chlorhexidine and Chlorhexidine Associated with Calcium Hydroxide
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DOI：
10.1016/j.joen.2008.08.032
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发表时间：
2008-12-01
影响因子：
4.2
通讯作者：
Pecora, Jesus Djalma
中科院分区：
文献类型：
--
作者：
Barbin, Luiz Eduardo;Saquy, Paulo Cesar;Pecora, Jesus Djalma
本研究的目的是确定氯己定（CHX）单独或CHX与氢氧化钙在不同时间点混合后是否会产生对氯苯胺（PCA）和/或活性氧（ROS）。质谱法检测0.2% CHX和Ca(OH)2与0.2% CHX混合样品中的PCA。用高效液相色谱法确定CHX与Ca(OH)2的混合物中存在CHX。混合后立即、第7天和第14天后分析样品。在实验期间，样品保持在36.5℃和95%的相对湿度。14天后，在0.2% CHX溶液中检测PCA。CHX与Ca(CH)2的混合物在所有时间点释放ROS，但由于CHX的立即降解，混合物中没有CHX的痕迹。[J] end 2008;34:1508-1514。
The aim of this study was to determine whether para-chloroaniline (PCA) and/or reactive oxygen species (ROS) are generated by chlorhexidine (CHX) alone or after CHX is mixed with calcium hydroxide at different time points. Mass spectrometry was performed to detect PCA in samples of 0.2% CHX and Ca(OH)2 mixed with 0.2% CHX. High-performance liquid chromatography was used to confirm the presence of CHX in the mixture with Ca(OH)2. The samples were analyzed immediately after mixing and after 7 and 14 days. During the intervals of the experiment, the samples were maintained at 36.5 degrees C and 95% relative humidity. PCA was detected in the 0.2% CHX solution after 14 days. The mixture of CHX with Ca(CH)2 liberated ROS at all time points, but no traces of CHX were present in the mixture as a result of immediate degradation of the CHX. (J Endod 2008;34:1508-1514)