Complete mitochondrial genome sequence of Brassica oxyrrhina and comparative analysis of the region containing orf108, a male sterility gene for mustard (Brassica juncea), among B. oxyrrhina, Diplotaxis erucoides and Sinapis species.
Complete mitochondrial genome sequence of Brassica oxyrrhina and comparative analysis of the region containing orf108, a male sterility gene for mustard (Brassica juncea), among B. oxyrrhina, Diplotaxis erucoides and Sinapis species.
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尖头甘蓝线粒体基因组全序列，以及尖头甘蓝、芥菜和芥菜种芥菜雄性不育基因 orf108 区域的比较分析。
DOI：
10.1111/pbr.12710
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发表时间：
2019
期刊：
影响因子：
2
通讯作者：
Yamagishi H.
中科院分区：
文献类型：
--
作者：
Mukai A;Jikuya M;Tsujimura M;Terachi T;Yamagishi H.
迄今为止，几种野生物种的细胞质已被引入芥菜中，用于诱导细胞质雄性不育（CMS）。 isorf108是CMS的致病基因之一，广泛分布于包括尖头甘蓝在内的十字花科植物中。为了进一步了解orf108的起源，我们组装了B的完整线粒体基因组序列。尖鼻。我们还确定了 atp1 上游的 DNA 序列，包括 orf108forD。芥子和五个芥子种。 Theorf108 被明确放置在 B 的线粒体基因组中。 oxyrina 由 247,936 bp 组成。 InS。 alba，之前报道拥有者f108，该基因不存在，而Sinapis arvensis和Sinapis turgida则含有dorf108。与 D 相比，两个芥属物种中的 Theorf108 序列显示出新的核苷酸取代。芥菜。此外，Northern 杂交表明，orf108mRNA 在这两个物种中的加工过程与 B 中的相似。尖鼻。结果阐明了芥子orf108-apt1区域的种间分化。
To date, several cytoplasms of wild species have been introduced toBrassica juncea, for inducing cytoplasmic male sterility (CMS). One of the causal genes of CMS isorf108, which is widely distributed in Brassicaceae includingBrassica oxyrrhina. To further understand the origin oforf108, we assembled the complete mitochondrial genome sequence ofB. oxyrrhina. We also determined the DNA sequences upstream ofatp1includingorf108forD. erucoidesand fiveSinapisspecies. Theorf108was definitively placed in the mitochondrial genome ofB. oxyrrhinaconsisting of 247,936 bp. InS. alba, previously reported to possessorf108, the gene was not present, whereasSinapis arvensisandSinapis turgidacontainedorf108. Theorf108sequence in the twoSinapisspecies showed a novel nucleotide substitution compared toD. erucoides. Northern hybridization suggested, furthermore, thatorf108mRNA was processed in the two species similarly to that inB. oxyrrhina. The results clarified the interspecific differentiation oforf108‐apt1region inSinapis.