8DSS-Promoted Remineralization of Initial Enamel Caries In Vitro
8DSS-Promoted Remineralization of Initial Enamel Caries In Vitro
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DOI：
10.1177/0022034514522815
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发表时间：
2014-02
影响因子：
7.6
通讯作者：
Y. Yang;X. Lv;W. Shi;J.Y. Li;D.X. Li;X.D. Zhou;L.L. Zhang
中科院分区：
文献类型：
--
作者：
Y. Yang;X. Lv;W. Shi;J.Y. Li;D.X. Li;X.D. Zhou;L.L. Zhang
含有8个重复天冬氨酸-丝氨酸-丝氨酸（8DSS）的肽已被证明能促进磷酸钙从溶液进入人牙釉质的成核。在人工早期牙釉质龋的体外模型中，我们测试了8DSS促进脱矿牙釉质再矿化的能力。在牛牙釉质块中形成初始龋损，然后在25µM 8DSS， 1 g/L NaF（阳性对照）或单独缓冲液（阴性对照）存在下进行12 d的pH循环。用x射线光电子能谱法验证了8DSS的吸收。通过偏振光显微镜和横向显微放射照相分析pH循环前后病变体表层和不同深度的矿物质损失、病变深度和矿物质含量。pH循环后，8DSS样品中的矿物质损失明显低于仅使用缓冲液的样品，8DSS样品中的损伤深度也明显较浅。在从表层（30µm）到平均损伤深度（110µm）的范围内，经过8DSS处理的样品的矿物质含量明显高于仅经过缓冲处理的样品。8DSS处理的样品与NaF处理的样品之间无显著差异。这些发现表明8DSS具有促进脱矿牙釉质再矿化的潜力。
Peptides containing 8 repeats of aspartate-serine-serine (8DSS) have been shown to promote the nucleation of calcium phosphate from solution into human enamel. Here we tested the ability of 8DSS to promote the remineralization of demineralized enamel in an in vitro model of artificial early enamel caries. Initial caries lesions were created in bovine enamel blocks, which were then subjected to 12 d of pH cycling in the presence of 25 µM 8DSS, 1 g/L NaF (positive control) or buffer alone (negative control). Absorption of 8DSS was verified by X-ray photoelectron spectroscopy. Mineral loss, lesion depth, and mineral content at the surface layer and at different depths of the lesion body were analyzed before and after pH cycling by polarized light microscopy and transverse microradiography. Mineral loss after pH cycling was significantly lower in the 8DSS samples than in the buffer-only samples, and lesions in the 8DSS samples were significantly less deep. Samples treated with 8DSS showed significantly higher mineral content than buffer-only samples in the region extending from the surface layer (30 µm) to the average lesion depth (110 µm). No significant differences were found between the samples treated with 8DSS and those treated with NaF. These findings suggest that 8DSS has the potential to promote remineralization of demineralized enamel.