A Novel Poly(3-Hydroxybutyrate)-Degrading Streptoverticillium Kashmirense AF1 Isolated from Soil and Purification of Phb-Depolymerase
A Novel Poly(3-Hydroxybutyrate)-Degrading Streptoverticillium Kashmirense AF1 Isolated from Soil and Purification of Phb-Depolymerase
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从土壤中分离出新型聚（3-羟基丁酸酯）降解链轮丝菌克什米尔 AF1 及 Phb 解聚酶的纯化
DOI：
10.1556/abiol.59.2008.4.9
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发表时间：
2008
影响因子：
--
通讯作者：
Safia Ahmed
中科院分区：
文献类型：
--
作者：
A. A. Shah;F. Hasan;A. Hameed;Safia Ahmed
从土壤中分离到一株能降解聚3-羟基丁酸酯（PHB）的新菌株。这种细菌被鉴定为克什米尔链黄霉AF1，在固体和液体矿物盐培养基中都分泌PHB解聚合酶，其中含有聚3-羟基丁酸盐作为唯一碳源。pH值为8和7，温度为45°C，底物浓度为1%，乳糖作为附加碳源存在时，PHB解聚合酶的最佳产量。细胞外PHB解聚合酶采用Sephadex G-75凝胶渗透层析纯化。通过SDS-PAGE测定，克什米尔链杆菌AF1产生两种分子量分别为37和45 kDa的PHB解聚合酶。通过Sturm试验，用重量法计算了试验和对照的干细胞质量和CO_2含量的差异，表明了克什米尔链霉菌AF1的降解能力。
A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 °C, 1% substrate concentration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75. The Streptoverticillium kashmirense AF1 produced two types of PHB depolymerases having molecular weights of about 37 and 45 kDa as determined by SDS-PAGE. The difference in dry cell mass and amount of CO_2 evolved in the test and control calculated gravimetrically through Sturm test indicated the degradative capabilities of Streptoverticillium kashmirense AF1.