Polyethylene glycol-surfactant for lavage lung injury in rats
Polyethylene glycol-surfactant for lavage lung injury in rats
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DOI：
10.1203/01.pdr.0000182581.39561.01
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发表时间：
2005-11-01
影响因子：
3.6
通讯作者：
Taeusch, HW
中科院分区：
文献类型：
--
作者：
Dehority, W;Lu, KW;Taeusch, HW
在存在灭活物质的情况下，将离子和非离子水溶性聚合物添加到肺表面活性剂中可防止体外表面活性剂灭活，并改善几种肺损伤模型的肺功能。然而，最近的一份报告发现，当表面活性剂加聚乙二醇（PEG）用于治疗盐水肺灌洗引起的肺损伤时，效果相反。因此，我们研究了聚合物效应在盐水肺灌洗肺损伤模型中不太明显的原因。我们用来自牛肺的商业肺表面活性剂提取物（Survanta）（添加或不添加PEG）治疗灌洗肺损伤大鼠。用Survanta + PEG处理的组具有比用Survanta处理的组显著更高的静态死后肺体积。然而，与Survanta治疗组相比，Survanta + PEG治疗组有更多的气管液体，并且在动脉氧合方面没有显著益处，尽管我们使用了减少肺水肿的措施。血管内注射I-125标记的白蛋白后的测量结果证实，添加PEG增加了血管外肺水，并且呋塞米减轻了这种影响。我们的结论是，表面活性剂+ PEG混合物是有效的灌洗损伤比其他形式的肺损伤，因为增加血管外肺水。
Addition of ionic and nonionic water-soluble polymers to pulmonary surfactants in the presence of inactivating substances prevents surfactant inactivation in vitro and improves lung function in several models of lung injury. However, a recent report found opposite effects when surfactant plus polyethylene glycol (PEG) was used to treat lung injury caused by saline lung lavage. Therefore, we examined the reasons why the polymer effect is less evident in the saline lung lavage lung injury model. We treated rats with lavage lung injury with a commercial lung surfactant extract derived from bovine lung (Survanta) with or without addition of PEG. Groups treated with Survanta + PEG had significantly higher static post mortem lung volumes than groups treated with Survanta. However, groups treated with Survanta + PEG had more tracheal fluid and no significant benefit in arterial oxygenation compared with the group treated with Survanta, despite our use of measures to reduce pulmonary edema. Measurements after intravascular injections of I-125-labeled albumin confirmed that addition of PEG increased extravascular lung water and that this effect is mitigated by furosemide. We conclude that surfactant + PEG mixtures are less effective in lavage injury than in other forms of lung injury because of increased extravascular lung water.