A comparative proteomic analysis of Bacillus coagulans in response to lactate stress during the production of l-lactic acid
A comparative proteomic analysis of Bacillus coagulans in response to lactate stress during the production of l-lactic acid
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DOI：
10.1007/s10529-014-1639-6
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发表时间：
2014-09
影响因子：
2.7
通讯作者：
Xiuwen Wang;J. Qin;Landong Wang;P. Xu
中科院分区：
文献类型：
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作者：
Xiuwen Wang;J. Qin;Landong Wang;P. Xu
乳酸盐对凝结芽孢杆菌2 -6的生长速率和最大生物量均有抑制作用，乳酸钠的抑制作用强于乳酸钙。B.使用结合质谱鉴定的二维电泳测定乳酸应激下的凝结物2 -6。在非应激、乳酸钙应激和乳酸钠应激条件下，检测到的蛋白质斑点数分别为1，571 ± 117、1，281 ± 231和904 ± 127。鉴定了乳酸胁迫下高表达的4个蛋白：乳酸脱氢酶、半胱氨酸合成酶A、醛/酮还原酶和核糖体蛋白L7/L12。因此，这些蛋白质是重建B的潜在目标。促凝剂，以促进其抵抗乳酸应激。
The growth rate and maximum biomass ofBacillus coagulans2–6 were inhibited by lactate; inhibition by sodium lactate was stronger than by calcium lactate. The differences of protein expressions byB. coagulans2–6 under the lactate stress were determined using two-dimensional electrophoresis coupled with mass spectrometric identification. Under the non-stress condition, calcium lactate stress and sodium lactate stress, the number of detected protein spots was 1,571 ± 117, 1,281 ± 231 and 904 ± 127, respectively. Four proteins with high expression under lactate stress were identified: lactate dehydrogenase, cysteine synthase A, aldo/keto reductase and ribosomal protein L7/L12. These proteins are thus potential targets for the reconstruction ofB. coagulansto promote its resistance to lactate stress.