Identification of two novel genes encoding 97- to 99-kilodalton outer membrane proteins of Chlamydia pneumoniae
Identification of two novel genes encoding 97- to 99-kilodalton outer membrane proteins of Chlamydia pneumoniae
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DOI：
10.1128/iai.67.1.375-383.1999
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发表时间：
1999-01-01
影响因子：
3.1
通讯作者：
Birkelund, S
中科院分区：
文献类型：
--
作者：
Knudsen, K;Madsen, AS;Birkelund, S
克隆并测序了两个编码相互相似的 97-至 99-kDa 肺炎衣原体 VR1310 外膜蛋白（Omp4 和 Omp5）的基因。这些蛋白质被证明是肺炎衣原体外膜复合物的组成部分，并且推导的氨基酸序列与由鹦鹉热衣原体和沙眼衣原体基因家族编码的推定外膜蛋白质的氨基酸序列相似。通过使用针对纯化的重组Omp4的单特异性多克隆抗体，显示在不加热的情况下，蛋白质在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中以65至75kDa迁移，免疫电镜显示Omp4表位暴露于肺炎衣原体原体、网状体和外膜复合体表面，肺炎衣原体基因家族编码的蛋白似乎是实验感染小鼠的显性抗原。
Two genes encoding 97- to 99-kDa Chlamydia pneumoniae VR1310 outer membrane proteins (Omp4 and Omp5) with mutual similarity were cloned and sequenced. The proteins were shown to be constituents of the C. pneumoniae outer membrane complex, and the deduced amino acid sequences were similar to those of putative outer membrane proteins encoded by the Chlamydia psittaci and Chlamydia trachomatis gene families, By use of a monospecific polyclonal antibody against purified recombinant Omp4, it was shown that without heating, the protein migrated at 65 to 75 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Immunoelectron microscopy showed that epitopes of Omp4 were exposed on the surface of C, pneumoniae elementary bodies, reticulate bodies, and outer membrane complex, Proteins encoded by the C, pneumoniae gene family seem to be dominant antigens in experimentally infected mice.