QUANTITATIVE SINGLE CELL ANALYSIS AND SORTING
QUANTITATIVE SINGLE CELL ANALYSIS AND SORTING
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DOI：
10.1126/science.905822
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发表时间：
1977-01-01
期刊：
影响因子：
56.9
通讯作者：
WHEELESS, LL
中科院分区：
文献类型：
--
作者：
HORAN, PK;WHEELESS, LL
细胞的定量多参数流动分析和分选是生物医学研究和临床医学中的重要新技术之一。能够以非常高的速度获得单细胞的测量结果，并对这些细胞进行分类以进行进一步分析，这使得生物研究人员能够提出迄今为止无法接近的问题，因为不存在提供所需数据的分析方法。本文介绍了流式细胞仪的技术方面及其应用，包括一些更具创新性的新方法。
Quantitative multiparameter flow analysis and sorting of cells is emerging as one of the important new technologies in biological and biomedical research and clinical medicine. The ability to obtain measurements on single cells at very high rates and to sort these cells for further analysis has enabled biological researchers to ask questions that heretofore were not approachable because analytical methods did not exist to provide the needed data. This article describes the technical aspects of flow cytometric instrumentation and its applications, including some of the more innovative new approaches.