Ovulation-inducing factor in the seminal plasma of alpacas and llamas
Ovulation-inducing factor in the seminal plasma of alpacas and llamas
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DOI：
10.1095/biolreprod.105.040097
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发表时间：
2005-09-01
影响因子：
3.6
通讯作者：
Singh, J
中科院分区：
文献类型：
--
作者：
Adams, GP;Ratto, MH;Singh, J
进行了研究，以记录羊驼（实验1）和美洲驼（实验2）精浆中存在的排卵诱导因子，并确定该效应是否通过垂体介导（实验3）。在实验1中，雌性羊驼（n = 14/组）给予羊驼精浆或生理盐水肌肉注射或宫内输注。只有注射了精浆的羊驼。排卵率（13/ 14，93%; P < 0.01）。在实验2中，在精浆处理后平均29.3 +/- 0.7小时，在9/10（90%）的美洲驼中检测到排卵。血浆孕酮浓度在治疗后第9天达到最大值，在治疗后第12天达到最低值。在实验3中，给雌性大羊驼肌肉注射大羊驼精浆、GnRH或生理盐水，排卵率分别为6/6、5/ 6和0/6头（P < 0.001）。促性腺激素释放激素（GnRH）和精浆处理后15 min和75 min，血浆LH浓度分别开始升高（P < 0.01）。精浆组血浆LH持续升高时间较长（P < 0.05），8h后仍未降至治疗前水平。精浆组的黄体较促性腺激素释放激素组更长、直径更大（P = 0.1），血浆孕酮浓度是促性腺激素释放激素组的2倍（P < 0.01）。结果证明了羊驼和美洲驼精浆中存在一种强效因子，可引起循环LH浓度激增，并诱导排卵和促黄体反应。
Studies were conducted to document the existence of an ovulation-inducing factor in the seminal plasma of alpacas (experiment 1) and llamas (experiment 2) and to determine if the effect is mediated via the pituitary (experiment 3). In experiment 1, female alpacas (n = 14 per group) were given alpaca seminal plasma or saline intramuscularly or by intrauterine infusion. Only alpacas that were given seminal plasma i.m. ovulated (13/ 14, 93%; P < 0.01). In experiment 2, ovulation was detected in 9/10 (90%) llamas at a mean of 29.3 +/- 0.7 h after seminal plasma treatment. Plasma progesterone concentrations were maximal by Day 9 and were at nadir by Day 12 posttreatment. In experiment 3, female llamas were given llama seminal plasma, GnRH, or saline i.m., and ovulation was detected in 6/6, 5/ 6, and 0/6 llamas, respectively (P < 0.001). Treatment was followed by a surge (P < 0.01) in plasma LH concentration beginning 15 min and 75 min after treatment with GnRH and seminal plasma, respectively. Plasma LH remained elevated longer in the seminal plasma group (P < 0.05) and had not yet declined to pretreatment levels after 8 h. Compared with the GnRH group, corpus luteum tended to grow longer and to a greater diameter (P = 0.1) and plasma progesterone concentration was twice as high in the seminal plasma group (P < 0.01). Results document the existence of a potent factor in the seminal plasma of alpacas and llamas that elicited a surge in circulating concentrations of LH and induced an ovulatory and luteotropic response.