Soluble vascular endothelial growth factor receptor-1 contributes to the corneal antiangiogenic barrier
Soluble vascular endothelial growth factor receptor-1 contributes to the corneal antiangiogenic barrier
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DOI：
10.1136/bjo.2006.107417
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发表时间：
2007-04-01
影响因子：
4.1
通讯作者：
Caldwell, Ruth
中科院分区：
文献类型：
--
作者：
Ambati, Balamurali K.;Patterson, Emory;Caldwell, Ruth
目的：正常无血管角膜内的病理性新生血管形成是一种严重事件，可干扰正常视力。血管内皮生长因子（VEGF）的上调与眼睛中的新血管形成有关，表明维持低水平的VEGF对角膜无血管和完整视力很重要。本研究的目的是确定sVEGFR-1的表达谱和可能的贡献角膜avascular barrier.Design：实验病例系列研究VEGF和可溶性fms样酪氨酸激酶（sFlt）的水平在正常和新生血管化的人角膜。参会人员：4例正常人角膜，5例碱烧伤人角膜，3例无虹膜人角膜，1例眼瘢痕性类天疱疮和2例间质性角膜炎进行了检查。免疫沉淀法被用来证明sFlt-VEGF binding.Results：正常人角膜强烈表达sFlt在角膜上皮和弱的角膜基质接近利姆布斯。VEGF与正常人角膜中的sFlt结合。新生血管化的人角膜大大降低了sFlt的表达和显着减少VEGF结合sFlt.Conclusions：sFlt是高度表达的人角膜和正常隔离VEGF。
Purpose: Pathological neovascularisation within the normally avascular cornea is a serious event that can interfere with normal vision. Upregulation of vascular endothelial growth factor (VEGF) has been associated with neovascularisation in the eye, suggesting that maintaining low levels of VEGF is important for corneal avascularity and intact vision. This study aims to determine the expression profile and possible contribution of sVEGFR-1 to the corneal avascular barrier.Design: Experimental case series investigating VEGF and soluble fms-like tyrosine kinase (sFlt) levels in normal and neovascularised human corneas. Participants: Four normal human corneas, five human corneas with alkali burns, three human corneas with aniridia, one with ocular cicatricial pemphigoid and two with interstitial keratitis were examined.Methods: Western blot and immunohistochemical analyses were performed to determine sFlt and VEGF levels in normal and neovascularised human corneas. Immunoprecipitation was utilised to demonstrate sFlt-VEGF binding.Results: Normal human corneas strongly express sFlt in the corneal epithelium and weakly in the corneal stroma close to the limbus. VEGF is bound by sFlt in the normal human cornea. Neovascularised human corneas have greatly reduced expression of sFlt and significantly less VEGF bound by sFlt.Conclusions: sFlt is highly expressed in the human cornea and normally sequesters VEGF.